Reductions of myocardial Na-K-ATPase activity and ouabain binding sites in heart failure: Prevention by nadolol

T. H M Fan, Robert Frantz, H. Elam, S. Sakamoto, N. Imai, C. S. Liang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To study the changes in myocardial digitalis binding sites in heart failure, we measured myocardial ouabain binding sites, Na-K- adenosinetriphosphatase (ATPase) activity, and ventricular muscle mechanical responses to acetylstrophanthidin in dogs with right-heart failure (RHF) produced by tricuspid avulsion and pulmonary artery constriction. Sham- operated dogs were studied as the control. RHF produced a significant decrease in ouabain binding sites in the right and left ventricular myocardium, which was accompanied by a proportional decrease in Na-K-ATPase activity. However, RHF and sham-operated dogs did not differ in systemic hemodynamic or right ventricular trabeculate muscle isometric contractile responses to acetylstrophanthidin. To determine whether chronic β-adrenergic stimulation contributed to the development of Na-K-ATPase downregulation, we administered nadolol (40 mg/day) to a separate group of dogs during an early stage of RHF development. Nadolol effectively prevented the reduction of myocardial ouabain binding sites that occurred in RHF. Thus we conclude that myocardial ouabain binding sites and Na-K-ATPase activity are reduced in dogs with experimental heart failure and that these changes probably occur as a result of the attendant heightened sympathetic activity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume265
Issue number6 34-6
StatePublished - 1993
Externally publishedYes

Fingerprint

Nadolol
Ouabain
Adenosine Triphosphatases
Heart Failure
Binding Sites
Dogs
Muscles
Digitalis
Constriction
Adrenergic Agents
Pulmonary Artery
Myocardium
Down-Regulation
Hemodynamics

Keywords

  • congestive heart failure
  • nadolol
  • norepinephrine
  • ouabain binding sites
  • sodium-potassium pump

ASJC Scopus subject areas

  • Physiology

Cite this

Reductions of myocardial Na-K-ATPase activity and ouabain binding sites in heart failure : Prevention by nadolol. / Fan, T. H M; Frantz, Robert; Elam, H.; Sakamoto, S.; Imai, N.; Liang, C. S.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 265, No. 6 34-6, 1993.

Research output: Contribution to journalArticle

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