RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota

Jeffrey L. Winters, Alvaro A. Pineda, Lemuel D. Gorden, Sandra C. Bryant, L. Joseph Melton, Eleftherios C. Vamvakas, S. Breanndan Moore

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Abstract

BACKGROUND: While RBC antigen frequencies for whites of Northern European ancestry are known, the relative frequencies of RBC antibodies within this population have not been determined. The distribution of RBC alloantibodies by sex and age was studied, as were the immunogenicity of RBC antigens and the occurrence of RBC alloantibody clusters in a geographically defined population. STUDY DESIGN AND METHODS: RBC alloimmunization among patients and donors in Olmsted County, MN, was determined for the period from 1975 to 1995. Alloantibody frequencies were used to calculate the potency of each antigen relative to K. Cluster analysis was applied to the data to identify natural groupings of antibodies. RESULTS: The frequency and potency of 33 alloantibodies from 1345 alloimmunized subjects were estimated. The most frequent alloantibodies were E (20.8%), Lea (18.6%), K (14.7%), D (12.9%), Leb (9.4%), M (7.2%), P1 (6.7%), Fya (6.3%), C (6.8%), and c (3.5%). The most potent antigens were Wra (0.363), Cw (0.078), Lea (0.03), E (0.028), V (0.025), Jsa (0.023), Kpb (0.023), Goa (0.023), JMH (0.023), and Rd (0.023). Greater frequency of overall alloimmunization (M:F = 1:2.7), anti-D (p<0.0001), and anti-Lea (p = 0.003) was seen among females. Warm autoantibodies were more frequent among males with positive antibody screens (p<0.0001). No other gender differences were observed. Alloimmunization increased with age for K, Kpa, Fya, D, C, E, and warm autoantibodies. Frequencies of alloimmunization to Lea, Leb, M, and P1 decreased with age. The cluster analysis showed grouping of the antibodies to C and D as well as to Lea and Leb, but the other RBC alloantibodies did not form clusters. CONCLUSION: Less than 1 percent of residents tested had positive antibody screens. Anti-E and anti-Lea were more common than anti-K. Wra and Cw were more potent antigens than K. Most antibodies showed an increase in frequency with increasing age. Except for anti-C and -D and anti-Lea and -Leb, RBC alloantibodies did not occur in clusters.

Original languageEnglish (US)
Pages (from-to)1413-1420
Number of pages8
JournalTransfusion
Volume41
Issue number11
DOIs
StatePublished - 2001

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Isoantibodies
Antigens
Antibodies
Autoantibodies
Cluster Analysis
Dilatation and Curettage
Population
Tissue Donors

ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

Winters, J. L., Pineda, A. A., Gorden, L. D., Bryant, S. C., Melton, L. J., Vamvakas, E. C., & Moore, S. B. (2001). RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota. Transfusion, 41(11), 1413-1420. https://doi.org/10.1046/j.1537-2995.2001.41111413.x

RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota. / Winters, Jeffrey L.; Pineda, Alvaro A.; Gorden, Lemuel D.; Bryant, Sandra C.; Melton, L. Joseph; Vamvakas, Eleftherios C.; Moore, S. Breanndan.

In: Transfusion, Vol. 41, No. 11, 2001, p. 1413-1420.

Research output: Contribution to journalArticle

Winters, JL, Pineda, AA, Gorden, LD, Bryant, SC, Melton, LJ, Vamvakas, EC & Moore, SB 2001, 'RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota', Transfusion, vol. 41, no. 11, pp. 1413-1420. https://doi.org/10.1046/j.1537-2995.2001.41111413.x
Winters JL, Pineda AA, Gorden LD, Bryant SC, Melton LJ, Vamvakas EC et al. RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota. Transfusion. 2001;41(11):1413-1420. https://doi.org/10.1046/j.1537-2995.2001.41111413.x
Winters, Jeffrey L. ; Pineda, Alvaro A. ; Gorden, Lemuel D. ; Bryant, Sandra C. ; Melton, L. Joseph ; Vamvakas, Eleftherios C. ; Moore, S. Breanndan. / RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota. In: Transfusion. 2001 ; Vol. 41, No. 11. pp. 1413-1420.
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title = "RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota",
abstract = "BACKGROUND: While RBC antigen frequencies for whites of Northern European ancestry are known, the relative frequencies of RBC antibodies within this population have not been determined. The distribution of RBC alloantibodies by sex and age was studied, as were the immunogenicity of RBC antigens and the occurrence of RBC alloantibody clusters in a geographically defined population. STUDY DESIGN AND METHODS: RBC alloimmunization among patients and donors in Olmsted County, MN, was determined for the period from 1975 to 1995. Alloantibody frequencies were used to calculate the potency of each antigen relative to K. Cluster analysis was applied to the data to identify natural groupings of antibodies. RESULTS: The frequency and potency of 33 alloantibodies from 1345 alloimmunized subjects were estimated. The most frequent alloantibodies were E (20.8{\%}), Lea (18.6{\%}), K (14.7{\%}), D (12.9{\%}), Leb (9.4{\%}), M (7.2{\%}), P1 (6.7{\%}), Fya (6.3{\%}), C (6.8{\%}), and c (3.5{\%}). The most potent antigens were Wra (0.363), Cw (0.078), Lea (0.03), E (0.028), V (0.025), Jsa (0.023), Kpb (0.023), Goa (0.023), JMH (0.023), and Rd (0.023). Greater frequency of overall alloimmunization (M:F = 1:2.7), anti-D (p<0.0001), and anti-Lea (p = 0.003) was seen among females. Warm autoantibodies were more frequent among males with positive antibody screens (p<0.0001). No other gender differences were observed. Alloimmunization increased with age for K, Kpa, Fya, D, C, E, and warm autoantibodies. Frequencies of alloimmunization to Lea, Leb, M, and P1 decreased with age. The cluster analysis showed grouping of the antibodies to C and D as well as to Lea and Leb, but the other RBC alloantibodies did not form clusters. CONCLUSION: Less than 1 percent of residents tested had positive antibody screens. Anti-E and anti-Lea were more common than anti-K. Wra and Cw were more potent antigens than K. Most antibodies showed an increase in frequency with increasing age. Except for anti-C and -D and anti-Lea and -Leb, RBC alloantibodies did not occur in clusters.",
author = "Winters, {Jeffrey L.} and Pineda, {Alvaro A.} and Gorden, {Lemuel D.} and Bryant, {Sandra C.} and Melton, {L. Joseph} and Vamvakas, {Eleftherios C.} and Moore, {S. Breanndan}",
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T1 - RBC alloantibody specificity and antigen potency in Olmsted County, Minnesota

AU - Winters, Jeffrey L.

AU - Pineda, Alvaro A.

AU - Gorden, Lemuel D.

AU - Bryant, Sandra C.

AU - Melton, L. Joseph

AU - Vamvakas, Eleftherios C.

AU - Moore, S. Breanndan

PY - 2001

Y1 - 2001

N2 - BACKGROUND: While RBC antigen frequencies for whites of Northern European ancestry are known, the relative frequencies of RBC antibodies within this population have not been determined. The distribution of RBC alloantibodies by sex and age was studied, as were the immunogenicity of RBC antigens and the occurrence of RBC alloantibody clusters in a geographically defined population. STUDY DESIGN AND METHODS: RBC alloimmunization among patients and donors in Olmsted County, MN, was determined for the period from 1975 to 1995. Alloantibody frequencies were used to calculate the potency of each antigen relative to K. Cluster analysis was applied to the data to identify natural groupings of antibodies. RESULTS: The frequency and potency of 33 alloantibodies from 1345 alloimmunized subjects were estimated. The most frequent alloantibodies were E (20.8%), Lea (18.6%), K (14.7%), D (12.9%), Leb (9.4%), M (7.2%), P1 (6.7%), Fya (6.3%), C (6.8%), and c (3.5%). The most potent antigens were Wra (0.363), Cw (0.078), Lea (0.03), E (0.028), V (0.025), Jsa (0.023), Kpb (0.023), Goa (0.023), JMH (0.023), and Rd (0.023). Greater frequency of overall alloimmunization (M:F = 1:2.7), anti-D (p<0.0001), and anti-Lea (p = 0.003) was seen among females. Warm autoantibodies were more frequent among males with positive antibody screens (p<0.0001). No other gender differences were observed. Alloimmunization increased with age for K, Kpa, Fya, D, C, E, and warm autoantibodies. Frequencies of alloimmunization to Lea, Leb, M, and P1 decreased with age. The cluster analysis showed grouping of the antibodies to C and D as well as to Lea and Leb, but the other RBC alloantibodies did not form clusters. CONCLUSION: Less than 1 percent of residents tested had positive antibody screens. Anti-E and anti-Lea were more common than anti-K. Wra and Cw were more potent antigens than K. Most antibodies showed an increase in frequency with increasing age. Except for anti-C and -D and anti-Lea and -Leb, RBC alloantibodies did not occur in clusters.

AB - BACKGROUND: While RBC antigen frequencies for whites of Northern European ancestry are known, the relative frequencies of RBC antibodies within this population have not been determined. The distribution of RBC alloantibodies by sex and age was studied, as were the immunogenicity of RBC antigens and the occurrence of RBC alloantibody clusters in a geographically defined population. STUDY DESIGN AND METHODS: RBC alloimmunization among patients and donors in Olmsted County, MN, was determined for the period from 1975 to 1995. Alloantibody frequencies were used to calculate the potency of each antigen relative to K. Cluster analysis was applied to the data to identify natural groupings of antibodies. RESULTS: The frequency and potency of 33 alloantibodies from 1345 alloimmunized subjects were estimated. The most frequent alloantibodies were E (20.8%), Lea (18.6%), K (14.7%), D (12.9%), Leb (9.4%), M (7.2%), P1 (6.7%), Fya (6.3%), C (6.8%), and c (3.5%). The most potent antigens were Wra (0.363), Cw (0.078), Lea (0.03), E (0.028), V (0.025), Jsa (0.023), Kpb (0.023), Goa (0.023), JMH (0.023), and Rd (0.023). Greater frequency of overall alloimmunization (M:F = 1:2.7), anti-D (p<0.0001), and anti-Lea (p = 0.003) was seen among females. Warm autoantibodies were more frequent among males with positive antibody screens (p<0.0001). No other gender differences were observed. Alloimmunization increased with age for K, Kpa, Fya, D, C, E, and warm autoantibodies. Frequencies of alloimmunization to Lea, Leb, M, and P1 decreased with age. The cluster analysis showed grouping of the antibodies to C and D as well as to Lea and Leb, but the other RBC alloantibodies did not form clusters. CONCLUSION: Less than 1 percent of residents tested had positive antibody screens. Anti-E and anti-Lea were more common than anti-K. Wra and Cw were more potent antigens than K. Most antibodies showed an increase in frequency with increasing age. Except for anti-C and -D and anti-Lea and -Leb, RBC alloantibodies did not occur in clusters.

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