Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Jean M. Lacey, Mark J. Magera, Dietrich Matern, John F. O'Brien

Research output: Contribution to conferencePaper

Abstract

A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

Original languageEnglish (US)
Pages565-566
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

Fingerprint Dive into the research topics of 'Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)'. Together they form a unique fingerprint.

  • Cite this

    Lacey, J. M., Magera, M. J., Matern, D., & O'Brien, J. F. (2002). Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 565-566. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.