Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Jean M. Lacey; Mark J. Magera; Dietrich Matern; John F. O'Brien

Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Jean M. Lacey, Mark J. Magera, Dietrich Matern, John F. O'Brien

Laboratory Medicine and Pathology

Research output: Contribution to conference › Paper › peer-review

Abstract

A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

Original language	English (US)
Pages	565-566
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Lacey, J. M., Magera, M. J., Matern, D., & O'Brien, J. F. (2002). Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 565-566. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). / Lacey, Jean M.; Magera, Mark J.; Matern, Dietrich et al.
2002. 565-566 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

Lacey, JM, Magera, MJ, Matern, D & O'Brien, JF 2002, 'Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 565-566.

Lacey, Jean M. ; Magera, Mark J. ; Matern, Dietrich et al. / Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.

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abstract = "A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.",

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AU - Matern, Dietrich

AU - O'Brien, John F.

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N2 - A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

AB - A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

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Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Abstract

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