Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Jean M. Lacey, Mark J. Magera, Dietrich Matern, John F. O'Brien

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages565-566
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

thiopurine methyltransferase
Liquid chromatography
Mass spectrometry
Blood
Cells
Recovery
6-methylthiopurine
Liquids

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Lacey, J. M., Magera, M. J., Matern, D., & O'Brien, J. F. (2002). Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 565-566)

Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). / Lacey, Jean M.; Magera, Mark J.; Matern, Dietrich; O'Brien, John F.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 565-566.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lacey, JM, Magera, MJ, Matern, D & O'Brien, JF 2002, Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 565-566, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Lacey JM, Magera MJ, Matern D, O'Brien JF. Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 565-566
Lacey, Jean M. ; Magera, Mark J. ; Matern, Dietrich ; O'Brien, John F. / Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 565-566
@inproceedings{b9c42406463e4f8da2acc6201dfc2711,
title = "Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)",
abstract = "A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95{\%} (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.",
author = "Lacey, {Jean M.} and Magera, {Mark J.} and Dietrich Matern and O'Brien, {John F.}",
year = "2002",
language = "English (US)",
pages = "565--566",
booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

}

TY - GEN

T1 - Rapid quantitation of 6-methylmercaptopurine (6-MMP) for the determination of thiopurine methyltransferase (TPMT) activity by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

AU - Lacey, Jean M.

AU - Magera, Mark J.

AU - Matern, Dietrich

AU - O'Brien, John F.

PY - 2002

Y1 - 2002

N2 - A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

AB - A quantitative method using liquid chromatograpy and tandem mass spectrometry (LC-MS/MS) for the measurement of 6-methylmercaptopurine (6-MMP) in RBC is discussed. 0.1 mL RBC lysate was spiked with 1 nmol/mL of the internal standard 6-methylthioguanine (6-MTG) in the analysis. The mixture was incubated with 6-MP and cold SAM to determine 6-MMP formation. The recovery of 6-MMP added to RBC lysate at 2.7, 5.4, and 13.5 nmol/mL was>95% (N=108). The rate of formation of 6-MMP is an indication of the thiopurine methyltransferase (TPMT) activity present in a patient's red blood cell (RBC) lysate.

UR - http://www.scopus.com/inward/record.url?scp=2442664204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442664204&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442664204

SP - 565

EP - 566

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

ER -