Rapid conjugation of nanoparticles, proteins and siRNAs to microbubbles by strain-promoted click chemistry for ultrasound imaging and drug delivery

Xifeng Liu, Ping Gong, Pengfei Song, Feng Xie, A. Lee Miller, Shigao D Chen, Lichun Lu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new strategy using catalyst-free strain-promoted alkyne-azide cycloaddition (SPAAC) "click" chemistry for the ligation of anti-cancer drug-loaded nanoparticles, functionalized proteins, and siRNA conjugated micelles to microbubbles (MB) was established. The results showed fast ligation within 5 min without sacrificing microbubble size and density. The ultrasound test showed good imaging abilities of the microbubbles after functionalization. This microbubble-therapeutic SPAAC "click" conjugation developed in the current study involves no toxic catalyst or initiator, has ultra-fast reaction speed, and is versatile for the ligation of various anti-cancer or therapeutic agents to microbubbles. These advantages render the SPAAC click strategy promising for broad applications in ultrasound-guided imaging and therapeutic delivery.

Original languageEnglish (US)
Pages (from-to)705-717
Number of pages13
JournalPolymer Chemistry
Volume10
Issue number6
DOIs
StatePublished - Feb 14 2019

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Click Chemistry
Microbubbles
Alkynes
Azides
Cycloaddition
Drug delivery
Nanoparticles
Ultrasonography
Ultrasonics
Proteins
Cycloaddition Reaction
Imaging techniques
Ligation
Pharmaceutical Preparations
Catalysts
Poisons
Micelles
Small Interfering RNA
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Polymers and Plastics
  • Organic Chemistry

Cite this

Rapid conjugation of nanoparticles, proteins and siRNAs to microbubbles by strain-promoted click chemistry for ultrasound imaging and drug delivery. / Liu, Xifeng; Gong, Ping; Song, Pengfei; Xie, Feng; Miller, A. Lee; Chen, Shigao D; Lu, Lichun.

In: Polymer Chemistry, Vol. 10, No. 6, 14.02.2019, p. 705-717.

Research output: Contribution to journalArticle

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