TY - GEN
T1 - Rapid amplification of femtograms of DNA with high purity in digital microfluidics for sequencing
AU - Liu, Yuguang
AU - Jeraldo, Patricio
AU - Mendes-Soares, Helena
AU - Masters, Thao
AU - Nelson, Heidi
AU - Patel, Robin
AU - Chia, Nicholas
AU - Walther-Antonio, Marina
N1 - Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Whole genome sequencing is a promising tool for untargeted bacterial detection in various infectious diseases. It often relies on multi-step and time-consuming processes from sample preparation to the final analysis. More often, samples can contain a low abundance of bacterial DNA which require amplification with high purity. Here, we developed a digital microfluidic device for whole genome amplification and library preparation of as low as 10 femtograms of Corynebacterium glutamicum DNA within 3 hours.
AB - Whole genome sequencing is a promising tool for untargeted bacterial detection in various infectious diseases. It often relies on multi-step and time-consuming processes from sample preparation to the final analysis. More often, samples can contain a low abundance of bacterial DNA which require amplification with high purity. Here, we developed a digital microfluidic device for whole genome amplification and library preparation of as low as 10 femtograms of Corynebacterium glutamicum DNA within 3 hours.
KW - Bacterial DNA detection
KW - Electrowetting
KW - Microfluidic diagnostics
UR - http://www.scopus.com/inward/record.url?scp=85098247561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098247561&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098247561
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1195
EP - 1196
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -