Rapid amplification of femtograms of DNA with high purity in digital microfluidics for sequencing

Yuguang Liu, Patricio Jeraldo, Helena Mendes-Soares, Thao Masters, Heidi Nelson, Robin Patel, Nicholas Chia, Marina Walther-Antonio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Whole genome sequencing is a promising tool for untargeted bacterial detection in various infectious diseases. It often relies on multi-step and time-consuming processes from sample preparation to the final analysis. More often, samples can contain a low abundance of bacterial DNA which require amplification with high purity. Here, we developed a digital microfluidic device for whole genome amplification and library preparation of as low as 10 femtograms of Corynebacterium glutamicum DNA within 3 hours.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1195-1196
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • Bacterial DNA detection
  • Electrowetting
  • Microfluidic diagnostics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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