TY - GEN
T1 - Rapid amplification of femtograms of DNA with high purity in digital microfluidics for sequencing
AU - Liu, Yuguang
AU - Jeraldo, Patricio
AU - Mendes-Soares, Helena
AU - Masters, Thao
AU - Nelson, Heidi
AU - Patel, Robin
AU - Chia, Nicholas
AU - Walther-Antonio, Marina
N1 - Funding Information:
This work was supported by the Ivan Bowen Family Foundation. In addition, we thank the Microbiome Program and the Center for Individualized Medicine at Mayo Clinic for their support, and Dr. Alexander Revzin at Mayo Clinic for granting us the access to his microfabrication facilities.
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Whole genome sequencing is a promising tool for untargeted bacterial detection in various infectious diseases. It often relies on multi-step and time-consuming processes from sample preparation to the final analysis. More often, samples can contain a low abundance of bacterial DNA which require amplification with high purity. Here, we developed a digital microfluidic device for whole genome amplification and library preparation of as low as 10 femtograms of Corynebacterium glutamicum DNA within 3 hours.
AB - Whole genome sequencing is a promising tool for untargeted bacterial detection in various infectious diseases. It often relies on multi-step and time-consuming processes from sample preparation to the final analysis. More often, samples can contain a low abundance of bacterial DNA which require amplification with high purity. Here, we developed a digital microfluidic device for whole genome amplification and library preparation of as low as 10 femtograms of Corynebacterium glutamicum DNA within 3 hours.
KW - Bacterial DNA detection
KW - Electrowetting
KW - Microfluidic diagnostics
UR - http://www.scopus.com/inward/record.url?scp=85098247561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098247561&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098247561
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1195
EP - 1196
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -