Quantitative, video-based histochemistry to measure regional effects of anticholinesterase pesticides in rat brain

Pamela I. Hammond, Tanya Jelacic, Stephanie Padilla, William Stephen Brimijoin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Acetylcholinesterase histochemistry was coupled to inexpensive and widely available apparatus for video microscopy and densitometry to study enzyme activity and inhibition in different parts of the rat brain. Quantitative histochemistry, under properly defined conditions, yielded an output that increased linearly with incubation time and section thickness and was a smooth hyperbolic function of substrate concentration. The time-course of staining after in vivo exposure to eserine revealed no sign that carbamate-induced cholinesterase inhibition was readily reversed in vitro. Brains from rats treated either with a carbamate or an organophosphate anticholinesterase pesticide showed significant regional variation in cholinesterase inhibition. The histochemical data corresponded well with data from biochemical assays of acetylcholinesterase activity (overall correlation coefficient of absolute values, r = 0.95). Also, a comparison of assay types by two-way analysis of variance showed no significant main effect. These results support the conclusion that video-based histochemistry is suitable for detailed studies of developmental and toxicological influences on cholinesterases in multiple microscopic regions of the rat brain.

Original languageEnglish (US)
Pages (from-to)82-92
Number of pages11
JournalAnalytical Biochemistry
Volume241
Issue number1
DOIs
StatePublished - Oct 1 1996

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Cholinesterase Inhibitors
Cholinesterases
Pesticides
Rats
Brain
Carbamates
Acetylcholinesterase
Assays
Hyperbolic functions
Enzyme inhibition
Video Microscopy
Physostigmine
Organophosphates
Densitometry
Enzyme activity
Analysis of variance (ANOVA)
Toxicology
Analysis of Variance
Microscopic examination
Staining and Labeling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Quantitative, video-based histochemistry to measure regional effects of anticholinesterase pesticides in rat brain. / Hammond, Pamela I.; Jelacic, Tanya; Padilla, Stephanie; Brimijoin, William Stephen.

In: Analytical Biochemistry, Vol. 241, No. 1, 01.10.1996, p. 82-92.

Research output: Contribution to journalArticle

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