Quantifying cardiac functions in embryonic and adult zebrafish

Tiffany Hoage, Yonghe Ding, Xiaolei H Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

39 Citations (Scopus)

Abstract

Zebrafish embryos have been extensively used to study heart development and cardiac function, mainly due to the unique embryology and genetics of this model organism. Since most human heart disease occurs during adulthood, adult zebrafish models of heart disease are being created to dissect mechanisms of the disease and discover novel therapies. However, due to its small heart size, the use of cardiac functional assays in the adult zebrafish has been limited. To address this bottleneck, the transparent fish line casper;Tg(cmlc2:nuDsRed) that has a red fluorescent heart can be used to document beating hearts in vivo and to quantify cardiac functions in adult zebrafish. Here, we describe our methods for quantifying shortening fraction and heart rate in embryonic zebrafish, as well as in the juvenile and adult casper;Tg(cmlc2:nuDsRed) fish. In addition, we describe the red blood cell flow rate assay that can be used to reflect cardiac function indirectly in zebrafish at any stage.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages11-20
Number of pages10
Volume843
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume843
ISSN (Print)10643745

Fingerprint

Zebrafish
Heart Diseases
Fishes
Embryology
Genetic Models
Embryonic Structures
Erythrocytes
Heart Rate

Keywords

  • Flow rate
  • Heart rate
  • Physiology
  • Shortening fraction
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hoage, T., Ding, Y., & Xu, X. H. (2012). Quantifying cardiac functions in embryonic and adult zebrafish. In Methods in Molecular Biology (Vol. 843, pp. 11-20). (Methods in Molecular Biology; Vol. 843). https://doi.org/10.1007/978-1-61779-523-7_2

Quantifying cardiac functions in embryonic and adult zebrafish. / Hoage, Tiffany; Ding, Yonghe; Xu, Xiaolei H.

Methods in Molecular Biology. Vol. 843 2012. p. 11-20 (Methods in Molecular Biology; Vol. 843).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hoage, T, Ding, Y & Xu, XH 2012, Quantifying cardiac functions in embryonic and adult zebrafish. in Methods in Molecular Biology. vol. 843, Methods in Molecular Biology, vol. 843, pp. 11-20. https://doi.org/10.1007/978-1-61779-523-7_2
Hoage T, Ding Y, Xu XH. Quantifying cardiac functions in embryonic and adult zebrafish. In Methods in Molecular Biology. Vol. 843. 2012. p. 11-20. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-523-7_2
Hoage, Tiffany ; Ding, Yonghe ; Xu, Xiaolei H. / Quantifying cardiac functions in embryonic and adult zebrafish. Methods in Molecular Biology. Vol. 843 2012. pp. 11-20 (Methods in Molecular Biology).
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