Quantification of Insulin Analogs by Liquid Chromatography–High-Resolution Mass Spectrometry

Erica M. Fatica, Nicholas E. Larkey, Ravinder J. Singh

Research output: Chapter in Book/Report/Conference proceedingChapter


Administration of exogenous insulin or insulin analogs is a common cause of hypoglycemia. The etiology of hypoglycemic episodes can be investigated by the measurement of insulin. However, frequently used synthetic insulin analogs show variable reactivity with immunoassays designed for the quantification of human insulin and may produce misleading results. To overcome this challenge, mass spectrometric methods can be applied to differentiate and accurately quantify insulin and its analogs. Here we describe a liquid chromatography–tandem high-resolution accurate mass (LC–HRAM) for the highly specific and independent quantification of insulin and its synthetic analogs including aspart, detemir, glargine, glulisine, and lispro. This method utilizes antibody affinity extraction followed by analysis on a high-resolution accurate mass spectrometer.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Hyperinsulinemia
  • Hypoglycemia
  • Insulin analogs
  • Liquid chromatography–high-resolution mass spectrometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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