Purification of exosome-like vesicles from urine

Christopher Y. Chen; Marie C. Hogan; Christopher J. Ward

doi:10.1016/B978-0-12-397945-2.00013-5

Purification of exosome-like vesicles from urine

Christopher Y. Chen, Marie C. Hogan, Christopher J. Ward

Nephrology and Hypertension

Research output: Chapter in Book/Report/Conference proceeding › Chapter

29 Scopus citations

Abstract

Urinary exosome-like vesicles (ELVs), 20-200 nm membrane-bound particles shed by renal epithelium, have been shown to interact with the primary cilia of distant epithelial cells of the nephron. These ELVs are emerging as an important source of protein, mRNA, and miRNA biomarkers to monitor renal disease. However, purification of ELVs is compromised by the presence of large amounts of the urinary protein Tamm-Horsfall Protein (THP). THP molecules oligomerize into long, double-helical strands several microns long. These linear assemblies form a three-dimensional gel which traps and sequesters ELVs in any centrifugation-based protocol. Here, we present a purification protocol that separates ELVs from THP and divides urinary ELVs into three distinct populations.

Original language	English (US)
Title of host publication	Cilia, Part A
Editors	Wallace F. Marshall
Pages	225-241
Number of pages	17
DOIs	https://doi.org/10.1016/B978-0-12-397945-2.00013-5
State	Published - Mar 26 2013

Publication series

Name	Methods in Enzymology
Volume	524
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Fingerprint

Keywords

Density centrifugation
Exosomes
Polycystin
Purification
Tamm Horsfall protein (THP)

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Cite this

Chen, C. Y., Hogan, M. C., & Ward, C. J. (2013). Purification of exosome-like vesicles from urine. In W. F. Marshall (Ed.), Cilia, Part A (pp. 225-241). (Methods in Enzymology; Vol. 524). https://doi.org/10.1016/B978-0-12-397945-2.00013-5

@inbook{edbee5e037704d159b5d4eb9a365f191,

title = "Purification of exosome-like vesicles from urine",

abstract = "Urinary exosome-like vesicles (ELVs), 20-200 nm membrane-bound particles shed by renal epithelium, have been shown to interact with the primary cilia of distant epithelial cells of the nephron. These ELVs are emerging as an important source of protein, mRNA, and miRNA biomarkers to monitor renal disease. However, purification of ELVs is compromised by the presence of large amounts of the urinary protein Tamm-Horsfall Protein (THP). THP molecules oligomerize into long, double-helical strands several microns long. These linear assemblies form a three-dimensional gel which traps and sequesters ELVs in any centrifugation-based protocol. Here, we present a purification protocol that separates ELVs from THP and divides urinary ELVs into three distinct populations.",

keywords = "Density centrifugation, Exosomes, Polycystin, Purification, Tamm Horsfall protein (THP)",

author = "Chen, {Christopher Y.} and Hogan, {Marie C.} and Ward, {Christopher J.}",

year = "2013",

month = mar

day = "26",

doi = "10.1016/B978-0-12-397945-2.00013-5",

language = "English (US)",

isbn = "9780123979452",

series = "Methods in Enzymology",

pages = "225--241",

editor = "Marshall, {Wallace F.}",

booktitle = "Cilia, Part A",

}

TY - CHAP

T1 - Purification of exosome-like vesicles from urine

AU - Chen, Christopher Y.

AU - Hogan, Marie C.

AU - Ward, Christopher J.

PY - 2013/3/26

Y1 - 2013/3/26

N2 - Urinary exosome-like vesicles (ELVs), 20-200 nm membrane-bound particles shed by renal epithelium, have been shown to interact with the primary cilia of distant epithelial cells of the nephron. These ELVs are emerging as an important source of protein, mRNA, and miRNA biomarkers to monitor renal disease. However, purification of ELVs is compromised by the presence of large amounts of the urinary protein Tamm-Horsfall Protein (THP). THP molecules oligomerize into long, double-helical strands several microns long. These linear assemblies form a three-dimensional gel which traps and sequesters ELVs in any centrifugation-based protocol. Here, we present a purification protocol that separates ELVs from THP and divides urinary ELVs into three distinct populations.

AB - Urinary exosome-like vesicles (ELVs), 20-200 nm membrane-bound particles shed by renal epithelium, have been shown to interact with the primary cilia of distant epithelial cells of the nephron. These ELVs are emerging as an important source of protein, mRNA, and miRNA biomarkers to monitor renal disease. However, purification of ELVs is compromised by the presence of large amounts of the urinary protein Tamm-Horsfall Protein (THP). THP molecules oligomerize into long, double-helical strands several microns long. These linear assemblies form a three-dimensional gel which traps and sequesters ELVs in any centrifugation-based protocol. Here, we present a purification protocol that separates ELVs from THP and divides urinary ELVs into three distinct populations.

KW - Density centrifugation

KW - Exosomes

KW - Polycystin

KW - Purification

KW - Tamm Horsfall protein (THP)

UR - http://www.scopus.com/inward/record.url?scp=84875279077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875279077&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-397945-2.00013-5

DO - 10.1016/B978-0-12-397945-2.00013-5

M3 - Chapter

C2 - 23498743

AN - SCOPUS:84875279077

SN - 9780123979452

T3 - Methods in Enzymology

SP - 225

EP - 241

BT - Cilia, Part A

A2 - Marshall, Wallace F.

ER -

Access to Document

10.1016/B978-0-12-397945-2.00013-5