Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance

Xitao Xie, Zhengping Yi, Sandeep Sinha, Meenu Madan, Benjamin P. Bowen, Paul R Langlais, Danjun Ma, Lawrence Mandarino, Christian Meyer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To provide a more global view of adipocyte changes in human insulin resistance by proteomics analyses. Methods: Baseline biopsies of abdominal subcutaneous adipose tissue were obtained from 23 subjects without diabetes. Euglycemic clamps were used to divide subjects into an insulin-resistant group (IR, N = 10) and an insulin-sensitive (IS, N = 13) group, which were of similar age and gender but unequal adiposity (greater in IR). Proteins of isolated adipocytes were quantified by mass spectrometry using normalized spectral abundance factors. Results: Of 1,245 proteins assigned, 30 were detected in at least 12 of the 23 subjects that differed significantly in abundance ≥1.5-fold between IR and IS. IR displayed a pattern of increased cytoskeletal proteins and decreased mitochondrial proteins and FABP4 and FABP5. In subgroup analyses of adiposity-matched subjects, several of these changes were less pronounced in IR, but the abundance of proteins related to lipid metabolism and the unfolded/misfolded protein response were significantly and unfavorably altered. Conclusions: These results confirm lower abundance of mitochondrial proteins and suggest increased cytoskeletal proteins and decreased FABP4 and FABP5 in subcutaneous adipocytes of typical IR individuals. Changes in proteins related to lipid metabolism and the unfolded/misfolded protein may discriminate IR and IS individuals of equal adiposity.

Original languageEnglish (US)
Pages (from-to)1506-1514
Number of pages9
JournalObesity
Volume24
Issue number7
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Fingerprint

Adipocytes
Proteomics
Insulin Resistance
Adiposity
Cytoskeletal Proteins
Mitochondrial Proteins
Lipid Metabolism
Proteins
Abdominal Subcutaneous Fat
Insulin
Unfolded Protein Response
Protein Unfolding
Glucose Clamp Technique
Mass Spectrometry
Biopsy

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Xie, X., Yi, Z., Sinha, S., Madan, M., Bowen, B. P., Langlais, P. R., ... Meyer, C. (2016). Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance. Obesity, 24(7), 1506-1514. https://doi.org/10.1002/oby.21528

Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance. / Xie, Xitao; Yi, Zhengping; Sinha, Sandeep; Madan, Meenu; Bowen, Benjamin P.; Langlais, Paul R; Ma, Danjun; Mandarino, Lawrence; Meyer, Christian.

In: Obesity, Vol. 24, No. 7, 01.07.2016, p. 1506-1514.

Research output: Contribution to journalArticle

Xie, X, Yi, Z, Sinha, S, Madan, M, Bowen, BP, Langlais, PR, Ma, D, Mandarino, L & Meyer, C 2016, 'Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance', Obesity, vol. 24, no. 7, pp. 1506-1514. https://doi.org/10.1002/oby.21528
Xie, Xitao ; Yi, Zhengping ; Sinha, Sandeep ; Madan, Meenu ; Bowen, Benjamin P. ; Langlais, Paul R ; Ma, Danjun ; Mandarino, Lawrence ; Meyer, Christian. / Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance. In: Obesity. 2016 ; Vol. 24, No. 7. pp. 1506-1514.
@article{3315800d0b744706816c22c0a13d92f9,
title = "Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance",
abstract = "Objective: To provide a more global view of adipocyte changes in human insulin resistance by proteomics analyses. Methods: Baseline biopsies of abdominal subcutaneous adipose tissue were obtained from 23 subjects without diabetes. Euglycemic clamps were used to divide subjects into an insulin-resistant group (IR, N = 10) and an insulin-sensitive (IS, N = 13) group, which were of similar age and gender but unequal adiposity (greater in IR). Proteins of isolated adipocytes were quantified by mass spectrometry using normalized spectral abundance factors. Results: Of 1,245 proteins assigned, 30 were detected in at least 12 of the 23 subjects that differed significantly in abundance ≥1.5-fold between IR and IS. IR displayed a pattern of increased cytoskeletal proteins and decreased mitochondrial proteins and FABP4 and FABP5. In subgroup analyses of adiposity-matched subjects, several of these changes were less pronounced in IR, but the abundance of proteins related to lipid metabolism and the unfolded/misfolded protein response were significantly and unfavorably altered. Conclusions: These results confirm lower abundance of mitochondrial proteins and suggest increased cytoskeletal proteins and decreased FABP4 and FABP5 in subcutaneous adipocytes of typical IR individuals. Changes in proteins related to lipid metabolism and the unfolded/misfolded protein may discriminate IR and IS individuals of equal adiposity.",
author = "Xitao Xie and Zhengping Yi and Sandeep Sinha and Meenu Madan and Bowen, {Benjamin P.} and Langlais, {Paul R} and Danjun Ma and Lawrence Mandarino and Christian Meyer",
year = "2016",
month = "7",
day = "1",
doi = "10.1002/oby.21528",
language = "English (US)",
volume = "24",
pages = "1506--1514",
journal = "Obesity",
issn = "1930-7381",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance

AU - Xie, Xitao

AU - Yi, Zhengping

AU - Sinha, Sandeep

AU - Madan, Meenu

AU - Bowen, Benjamin P.

AU - Langlais, Paul R

AU - Ma, Danjun

AU - Mandarino, Lawrence

AU - Meyer, Christian

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Objective: To provide a more global view of adipocyte changes in human insulin resistance by proteomics analyses. Methods: Baseline biopsies of abdominal subcutaneous adipose tissue were obtained from 23 subjects without diabetes. Euglycemic clamps were used to divide subjects into an insulin-resistant group (IR, N = 10) and an insulin-sensitive (IS, N = 13) group, which were of similar age and gender but unequal adiposity (greater in IR). Proteins of isolated adipocytes were quantified by mass spectrometry using normalized spectral abundance factors. Results: Of 1,245 proteins assigned, 30 were detected in at least 12 of the 23 subjects that differed significantly in abundance ≥1.5-fold between IR and IS. IR displayed a pattern of increased cytoskeletal proteins and decreased mitochondrial proteins and FABP4 and FABP5. In subgroup analyses of adiposity-matched subjects, several of these changes were less pronounced in IR, but the abundance of proteins related to lipid metabolism and the unfolded/misfolded protein response were significantly and unfavorably altered. Conclusions: These results confirm lower abundance of mitochondrial proteins and suggest increased cytoskeletal proteins and decreased FABP4 and FABP5 in subcutaneous adipocytes of typical IR individuals. Changes in proteins related to lipid metabolism and the unfolded/misfolded protein may discriminate IR and IS individuals of equal adiposity.

AB - Objective: To provide a more global view of adipocyte changes in human insulin resistance by proteomics analyses. Methods: Baseline biopsies of abdominal subcutaneous adipose tissue were obtained from 23 subjects without diabetes. Euglycemic clamps were used to divide subjects into an insulin-resistant group (IR, N = 10) and an insulin-sensitive (IS, N = 13) group, which were of similar age and gender but unequal adiposity (greater in IR). Proteins of isolated adipocytes were quantified by mass spectrometry using normalized spectral abundance factors. Results: Of 1,245 proteins assigned, 30 were detected in at least 12 of the 23 subjects that differed significantly in abundance ≥1.5-fold between IR and IS. IR displayed a pattern of increased cytoskeletal proteins and decreased mitochondrial proteins and FABP4 and FABP5. In subgroup analyses of adiposity-matched subjects, several of these changes were less pronounced in IR, but the abundance of proteins related to lipid metabolism and the unfolded/misfolded protein response were significantly and unfavorably altered. Conclusions: These results confirm lower abundance of mitochondrial proteins and suggest increased cytoskeletal proteins and decreased FABP4 and FABP5 in subcutaneous adipocytes of typical IR individuals. Changes in proteins related to lipid metabolism and the unfolded/misfolded protein may discriminate IR and IS individuals of equal adiposity.

UR - http://www.scopus.com/inward/record.url?scp=84976610590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976610590&partnerID=8YFLogxK

U2 - 10.1002/oby.21528

DO - 10.1002/oby.21528

M3 - Article

C2 - 27345962

AN - SCOPUS:84976610590

VL - 24

SP - 1506

EP - 1514

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 7

ER -