Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response

Vinuth N. Puttamallesh, Sreelakshmi K. Sreenivasamurthy, Pradeep Kumar Singh, H. C. Harsha, Anjali Ganjiwale, Shobha Broor, Akhilesh Pandey, Jayasuryan Narayana, T. S.Keshava Prasad

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.

Original languageEnglish (US)
Article number14
JournalClinical Proteomics
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Proteomics
Viruses
Chikungunya virus
Serum
Proteins
Aedes
Clusterin
Signal transduction
Apolipoproteins
RNA Viruses
Virus Diseases
Culicidae
Lipid Metabolism
Signal Transduction
Fever
RNA
Apoptosis
Molecules
Infection

Keywords

  • Arthritis
  • Hyponatremia
  • Itraq
  • Neuropathology
  • Quantitative proteomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Puttamallesh, V. N., Sreenivasamurthy, S. K., Singh, P. K., Harsha, H. C., Ganjiwale, A., Broor, S., ... Prasad, T. S. K. (2013). Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response. Clinical Proteomics, 10(1), [14]. https://doi.org/10.1186/1559-0275-10-14

Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response. / Puttamallesh, Vinuth N.; Sreenivasamurthy, Sreelakshmi K.; Singh, Pradeep Kumar; Harsha, H. C.; Ganjiwale, Anjali; Broor, Shobha; Pandey, Akhilesh; Narayana, Jayasuryan; Prasad, T. S.Keshava.

In: Clinical Proteomics, Vol. 10, No. 1, 14, 01.01.2013.

Research output: Contribution to journalArticle

Puttamallesh, VN, Sreenivasamurthy, SK, Singh, PK, Harsha, HC, Ganjiwale, A, Broor, S, Pandey, A, Narayana, J & Prasad, TSK 2013, 'Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response', Clinical Proteomics, vol. 10, no. 1, 14. https://doi.org/10.1186/1559-0275-10-14
Puttamallesh VN, Sreenivasamurthy SK, Singh PK, Harsha HC, Ganjiwale A, Broor S et al. Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response. Clinical Proteomics. 2013 Jan 1;10(1). 14. https://doi.org/10.1186/1559-0275-10-14
Puttamallesh, Vinuth N. ; Sreenivasamurthy, Sreelakshmi K. ; Singh, Pradeep Kumar ; Harsha, H. C. ; Ganjiwale, Anjali ; Broor, Shobha ; Pandey, Akhilesh ; Narayana, Jayasuryan ; Prasad, T. S.Keshava. / Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response. In: Clinical Proteomics. 2013 ; Vol. 10, No. 1.
@article{adba92f63ea44c26b24823f60961db3e,
title = "Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response",
abstract = "Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.",
keywords = "Arthritis, Hyponatremia, Itraq, Neuropathology, Quantitative proteomics",
author = "Puttamallesh, {Vinuth N.} and Sreenivasamurthy, {Sreelakshmi K.} and Singh, {Pradeep Kumar} and Harsha, {H. C.} and Anjali Ganjiwale and Shobha Broor and Akhilesh Pandey and Jayasuryan Narayana and Prasad, {T. S.Keshava}",
year = "2013",
month = "1",
day = "1",
doi = "10.1186/1559-0275-10-14",
language = "English (US)",
volume = "10",
journal = "Clinical Proteomics",
issn = "1542-6416",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response

AU - Puttamallesh, Vinuth N.

AU - Sreenivasamurthy, Sreelakshmi K.

AU - Singh, Pradeep Kumar

AU - Harsha, H. C.

AU - Ganjiwale, Anjali

AU - Broor, Shobha

AU - Pandey, Akhilesh

AU - Narayana, Jayasuryan

AU - Prasad, T. S.Keshava

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.

AB - Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.

KW - Arthritis

KW - Hyponatremia

KW - Itraq

KW - Neuropathology

KW - Quantitative proteomics

UR - http://www.scopus.com/inward/record.url?scp=84903733558&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903733558&partnerID=8YFLogxK

U2 - 10.1186/1559-0275-10-14

DO - 10.1186/1559-0275-10-14

M3 - Article

AN - SCOPUS:84903733558

VL - 10

JO - Clinical Proteomics

JF - Clinical Proteomics

SN - 1542-6416

IS - 1

M1 - 14

ER -