TY - JOUR
T1 - Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response
AU - Puttamallesh, Vinuth N.
AU - Sreenivasamurthy, Sreelakshmi K.
AU - Singh, Pradeep Kumar
AU - Harsha, H. C.
AU - Ganjiwale, Anjali
AU - Broor, Shobha
AU - Pandey, Akhilesh
AU - Narayana, Jayasuryan
AU - Prasad, T. S.Keshava
N1 - Funding Information:
We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics and All India Institute of Medical Sciences. Sreelakshmi K. Sreenivasamurthy is a recipient of Junior Research Fellowship from the University Grants Commission (UGC), Government of India. Vinuth N. Puttamallesh is funded by a Senior Research Fellowship from the DST-IDP research grant “Development of epitope based diagnostic gadget for detection of Mycobacterium tuberculosis in the Indian population” from the Department of Science and Technology (DST), Government of India to TS Keshava Prasad. TSK Prasad is also supported by a research grant on “Development of Infrastructure and a Computational Framework for Analysis of Proteomic Data” from DBT. HC Harsha is a Wellcome Trust-DBT India Alliance Early Career Fellow. We thank Agilent Technologies for instrumentation support.
PY - 2013
Y1 - 2013
N2 - Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.
AB - Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.
KW - Arthritis
KW - Hyponatremia
KW - Itraq
KW - Neuropathology
KW - Quantitative proteomics
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U2 - 10.1186/1559-0275-10-14
DO - 10.1186/1559-0275-10-14
M3 - Article
AN - SCOPUS:84903733558
SN - 1542-6416
VL - 10
JO - Clinical Proteomics
JF - Clinical Proteomics
IS - 1
M1 - 14
ER -