Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat

Yasuhiro H Ikeda, Yasuo Inoshima, Yasushi Kawaguchi, Ken Maeda, Mariko Kohmoto, Chieko Kai, Takayuki Miyazawa, Takeshi Mikami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Electrophoresis-mobility-shift assays with nuclear extracts from a feline renal cell line and a T-lymphoblastoid cell line revealed that the AP-1 and ATF sites of feline immunodeficiency virus (FIV) TM2 strain had similar protein-binding properties to those of FIV Petaluma strain and consensus sequences of AP-1 and ATF sites, and that nuclear factors binding to these sites differed between the two cell lines. Cross-competition and gel-supershift assays demonstrated that the AP-1 and ATF sites had similar protein-binding properties. The effects of internal deletions of AP-1 and/or ATF sites on the basal promoter activity were also examined. Although deletion of either site moderately reduced activity, a mutant deleted in both sites had dramatically activity. Therefore, we reduced suggest that these two sites co-operatively regulate transcriptional activity of the promoter.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalJournal of General Virology
Volume79
Issue number1
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Feline Immunodeficiency Virus
Terminal Repeat Sequences
Transcription Factor AP-1
Protein Binding
Cell Line
Felidae
Consensus Sequence
Electrophoretic Mobility Shift Assay
Electrophoresis
Gels
Binding Sites
Kidney

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Ikeda, Y. H., Inoshima, Y., Kawaguchi, Y., Maeda, K., Kohmoto, M., Kai, C., ... Mikami, T. (1998). Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat. Journal of General Virology, 79(1), 95-99.

Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat. / Ikeda, Yasuhiro H; Inoshima, Yasuo; Kawaguchi, Yasushi; Maeda, Ken; Kohmoto, Mariko; Kai, Chieko; Miyazawa, Takayuki; Mikami, Takeshi.

In: Journal of General Virology, Vol. 79, No. 1, 01.1998, p. 95-99.

Research output: Contribution to journalArticle

Ikeda, YH, Inoshima, Y, Kawaguchi, Y, Maeda, K, Kohmoto, M, Kai, C, Miyazawa, T & Mikami, T 1998, 'Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat', Journal of General Virology, vol. 79, no. 1, pp. 95-99.
Ikeda, Yasuhiro H ; Inoshima, Yasuo ; Kawaguchi, Yasushi ; Maeda, Ken ; Kohmoto, Mariko ; Kai, Chieko ; Miyazawa, Takayuki ; Mikami, Takeshi. / Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat. In: Journal of General Virology. 1998 ; Vol. 79, No. 1. pp. 95-99.
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