Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat

Yasuhiro Ikeda, Yasuo Inoshima, Yasushi Kawaguchi, Ken Maeda, Mariko Kohmoto, Chieko Kai, Takayuki Miyazawa, Takeshi Mikami

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Electrophoresis-mobility-shift assays with nuclear extracts from a feline renal cell line and a T-lymphoblastoid cell line revealed that the AP-1 and ATF sites of feline immunodeficiency virus (FIV) TM2 strain had similar protein-binding properties to those of FIV Petaluma strain and consensus sequences of AP-1 and ATF sites, and that nuclear factors binding to these sites differed between the two cell lines. Cross-competition and gel-supershift assays demonstrated that the AP-1 and ATF sites had similar protein-binding properties. The effects of internal deletions of AP-1 and/or ATF sites on the basal promoter activity were also examined. Although deletion of either site moderately reduced activity, a mutant deleted in both sites had dramatically activity. Therefore, we reduced suggest that these two sites co-operatively regulate transcriptional activity of the promoter.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalJournal of General Virology
Volume79
Issue number1
DOIs
StatePublished - Jan 1998

    Fingerprint

ASJC Scopus subject areas

  • Virology

Cite this