Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5

Jeong Jin Je, Hyun Taeg Shin, Seok Hee Chung, Jong Soo Lee, Sung Soo Kim, Hyun Dae Shin, Mi-Hyeon D Jang, Youn Jung Kim, Joo Ho Chung, Ee Hwa Kim, Chang Ju Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.

Original languageEnglish (US)
Pages (from-to)561-570
Number of pages10
JournalAmerican Journal of Chinese Medicine
Volume30
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

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Apoptosis
Cell Line
DNA Nucleotidylexotransferase
DNA Fragmentation
Neurodegenerative Diseases
Oxidative Stress
Central Nervous System
wuyaoshunqisan
thiazolyl blue

Keywords

  • Apoptosis
  • Hippocampus
  • Hydrogen Peroxide
  • Wuyaoshunqisan

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Je, J. J., Shin, H. T., Chung, S. H., Lee, J. S., Kim, S. S., Shin, H. D., ... Kim, C. J. (2002). Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5. American Journal of Chinese Medicine, 30(4), 561-570. https://doi.org/10.1142/S0192415X0200048X

Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5. / Je, Jeong Jin; Shin, Hyun Taeg; Chung, Seok Hee; Lee, Jong Soo; Kim, Sung Soo; Shin, Hyun Dae; Jang, Mi-Hyeon D; Kim, Youn Jung; Chung, Joo Ho; Kim, Ee Hwa; Kim, Chang Ju.

In: American Journal of Chinese Medicine, Vol. 30, No. 4, 2002, p. 561-570.

Research output: Contribution to journalArticle

Je, JJ, Shin, HT, Chung, SH, Lee, JS, Kim, SS, Shin, HD, Jang, M-HD, Kim, YJ, Chung, JH, Kim, EH & Kim, CJ 2002, 'Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5', American Journal of Chinese Medicine, vol. 30, no. 4, pp. 561-570. https://doi.org/10.1142/S0192415X0200048X
Je, Jeong Jin ; Shin, Hyun Taeg ; Chung, Seok Hee ; Lee, Jong Soo ; Kim, Sung Soo ; Shin, Hyun Dae ; Jang, Mi-Hyeon D ; Kim, Youn Jung ; Chung, Joo Ho ; Kim, Ee Hwa ; Kim, Chang Ju. / Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5. In: American Journal of Chinese Medicine. 2002 ; Vol. 30, No. 4. pp. 561-570.
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