Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5

Jeong Jin Je, Hyun Taeg Shin, Seok Hee Chung, Jong Soo Lee, Sung Soo Kim, Hyun Dae Shin, Mi Hyeon Jang, Youn Jung Kim, Joo Ho Chung, Ee Hwa Kim, Chang Ju Kim

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.

Original languageEnglish (US)
Pages (from-to)561-570
Number of pages10
JournalAmerican Journal of Chinese Medicine
Volume30
Issue number4
DOIs
StatePublished - 2002

Keywords

  • Apoptosis
  • Hippocampus
  • Hydrogen Peroxide
  • Wuyaoshunqisan

ASJC Scopus subject areas

  • Complementary and alternative medicine

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