Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5

Jeong Jin Je; Hyun Taeg Shin; Seok Hee Chung; Jong Soo Lee; Sung Soo Kim; Hyun Dae Shin; Mi Hyeon Jang; Youn Jung Kim; Joo Ho Chung; Ee Hwa Kim; Chang Ju Kim

doi:10.1142/S0192415X0200048X

Protective effects of Wuyaoshunqisan against H₂O ₂-induced apoptosis on hippocampal cell line HiB5

Jeong Jin Je, Hyun Taeg Shin, Seok Hee Chung, Jong Soo Lee, Sung Soo Kim, Hyun Dae Shin, Mi Hyeon Jang, Youn Jung Kim, Joo Ho Chung, Ee Hwa Kim, Chang Ju Kim

Neurosurgery

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H₂O₂-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H₂O₂ exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H₂O₂ exposure.

Original language	English (US)
Pages (from-to)	561-570
Number of pages	10
Journal	American Journal of Chinese Medicine
Volume	30
Issue number	4
DOIs	https://doi.org/10.1142/S0192415X0200048X
State	Published - 2002

Keywords

Apoptosis
Hippocampus
Hydrogen Peroxide
Wuyaoshunqisan

ASJC Scopus subject areas

Complementary and alternative medicine

Access to Document

10.1142/S0192415X0200048X

Cite this

@article{a334320dff0e45659020f99789349cb8,

title = "Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5",

abstract = "Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.",

keywords = "Apoptosis, Hippocampus, Hydrogen Peroxide, Wuyaoshunqisan",

author = "Je, {Jeong Jin} and Shin, {Hyun Taeg} and Chung, {Seok Hee} and Lee, {Jong Soo} and Kim, {Sung Soo} and Shin, {Hyun Dae} and Jang, {Mi Hyeon} and Kim, {Youn Jung} and Chung, {Joo Ho} and Kim, {Ee Hwa} and Kim, {Chang Ju}",

note = "Funding Information: This work was supported by a grant (PF002102-00) from Plant Diversity Research Center of 21st Frontier Research Program funded by the Korean Ministry of Science and Technology",

year = "2002",

doi = "10.1142/S0192415X0200048X",

language = "English (US)",

volume = "30",

pages = "561--570",

journal = "American Journal of Chinese Medicine",

issn = "0192-415X",

publisher = "World Scientific Publishing Co. Pte Ltd",

number = "4",

}

TY - JOUR

T1 - Protective effects of Wuyaoshunqisan against H2O 2-induced apoptosis on hippocampal cell line HiB5

AU - Je, Jeong Jin

AU - Shin, Hyun Taeg

AU - Chung, Seok Hee

AU - Lee, Jong Soo

AU - Kim, Sung Soo

AU - Shin, Hyun Dae

AU - Jang, Mi Hyeon

AU - Kim, Youn Jung

AU - Chung, Joo Ho

AU - Kim, Ee Hwa

AU - Kim, Chang Ju

N1 - Funding Information: This work was supported by a grant (PF002102-00) from Plant Diversity Research Center of 21st Frontier Research Program funded by the Korean Ministry of Science and Technology

PY - 2002

Y1 - 2002

N2 - Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.

AB - Oxidative stress has been implicated in the pathogenesis of different neurodegenerative disorders. To investigate the protective effects of Wuyaoshunqisan against H2O2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) method, flow cytometric analysis, and the DNA fragmentation assay were performed on cells of the hippocampal cell line HiB5. Through the morphological and biochemical analyses, it was shown that HiB5 cells treated with H2O2 exhibit classical apoptotic features, while the occurrence of such changes is reduced in cells pre-treated with Wuyaoshunqisan prior to H2O2 exposure.

KW - Apoptosis

KW - Hippocampus

KW - Hydrogen Peroxide

KW - Wuyaoshunqisan

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