Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells

Hyun Taeg Shin, Seok Hee Chung, Jong Soo Lee, Sung Soo Kim, Hyun Dae Shin, Mi-Hyeon D Jang, Min Chul Shin, Geon Ho Bahn, Eun Kyung Paik, Jae Hyung Park, Chang Ju Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.

Original languageEnglish (US)
Pages (from-to)675-686
Number of pages12
JournalAmerican Journal of Chinese Medicine
Volume31
Issue number5
DOIs
StatePublished - 2003
Externally publishedYes

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Apoptosis
N-Methylaspartate
Hydrogen Peroxide
Ions
Neurons
Cell Line
shenqi
aspartic acid receptor

Keywords

  • Corni fructus
  • Hippocampus
  • Hydrogen peroxide
  • N-methyl-D-aspartic acid receptor
  • Rehmanniae radix
  • Shenqi-wan

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells. / Shin, Hyun Taeg; Chung, Seok Hee; Lee, Jong Soo; Kim, Sung Soo; Shin, Hyun Dae; Jang, Mi-Hyeon D; Shin, Min Chul; Bahn, Geon Ho; Paik, Eun Kyung; Park, Jae Hyung; Kim, Chang Ju.

In: American Journal of Chinese Medicine, Vol. 31, No. 5, 2003, p. 675-686.

Research output: Contribution to journalArticle

Shin, HT, Chung, SH, Lee, JS, Kim, SS, Shin, HD, Jang, M-HD, Shin, MC, Bahn, GH, Paik, EK, Park, JH & Kim, CJ 2003, 'Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells', American Journal of Chinese Medicine, vol. 31, no. 5, pp. 675-686. https://doi.org/10.1142/S0192415X03001454
Shin, Hyun Taeg ; Chung, Seok Hee ; Lee, Jong Soo ; Kim, Sung Soo ; Shin, Hyun Dae ; Jang, Mi-Hyeon D ; Shin, Min Chul ; Bahn, Geon Ho ; Paik, Eun Kyung ; Park, Jae Hyung ; Kim, Chang Ju. / Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells. In: American Journal of Chinese Medicine. 2003 ; Vol. 31, No. 5. pp. 675-686.
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AU - Kim, Sung Soo

AU - Shin, Hyun Dae

AU - Jang, Mi-Hyeon D

AU - Shin, Min Chul

AU - Bahn, Geon Ho

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AU - Kim, Chang Ju

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