Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells

Hyun Taeg Shin; Seok Hee Chung; Jong Soo Lee; Sung Soo Kim; Hyun Dae Shin; Mi Hyeon Jang; Min Chul Shin; Geon Ho Bahn; Eun Kyung Paik; Jae Hyung Park; Chang Ju Kim

doi:10.1142/S0192415X03001454

Protective effect of Shenqi-wan against H₂O₂-induced apoptosis in hippocampal neuronal cells

Hyun Taeg Shin, Seok Hee Chung, Jong Soo Lee, Sung Soo Kim, Hyun Dae Shin, Mi Hyeon Jang, Min Chul Shin, Geon Ho Bahn, Eun Kyung Paik, Jae Hyung Park, Chang Ju Kim

Neurosurgery

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H₂O₂)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H₂O ₂ exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H₂O₂ exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H₂O₂-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.

Original language	English (US)
Pages (from-to)	675-686
Number of pages	12
Journal	American Journal of Chinese Medicine
Volume	31
Issue number	5
DOIs	https://doi.org/10.1142/S0192415X03001454
State	Published - 2003

Keywords

Corni fructus
Hippocampus
Hydrogen peroxide
N-methyl-D-aspartic acid receptor
Rehmanniae radix
Shenqi-wan

ASJC Scopus subject areas

Complementary and alternative medicine

Access to Document

10.1142/S0192415X03001454

Cite this

@article{f21c6b4b03c6488b9c27a6b7f6017be0,

title = "Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells",

abstract = "The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.",

keywords = "Corni fructus, Hippocampus, Hydrogen peroxide, N-methyl-D-aspartic acid receptor, Rehmanniae radix, Shenqi-wan",

author = "Shin, {Hyun Taeg} and Chung, {Seok Hee} and Lee, {Jong Soo} and Kim, {Sung Soo} and Shin, {Hyun Dae} and Jang, {Mi Hyeon} and Shin, {Min Chul} and Bahn, {Geon Ho} and Paik, {Eun Kyung} and Park, {Jae Hyung} and Kim, {Chang Ju}",

year = "2003",

doi = "10.1142/S0192415X03001454",

language = "English (US)",

volume = "31",

pages = "675--686",

journal = "American Journal of Chinese Medicine",

issn = "0192-415X",

publisher = "World Scientific Publishing Co. Pte Ltd",

number = "5",

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TY - JOUR

T1 - Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells

AU - Shin, Hyun Taeg

AU - Chung, Seok Hee

AU - Lee, Jong Soo

AU - Kim, Sung Soo

AU - Shin, Hyun Dae

AU - Jang, Mi Hyeon

AU - Shin, Min Chul

AU - Bahn, Geon Ho

AU - Paik, Eun Kyung

AU - Park, Jae Hyung

AU - Kim, Chang Ju

PY - 2003

Y1 - 2003

N2 - The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.

AB - The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.

KW - Corni fructus

KW - Hippocampus

KW - Hydrogen peroxide

KW - N-methyl-D-aspartic acid receptor

KW - Rehmanniae radix

KW - Shenqi-wan

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JO - American Journal of Chinese Medicine

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