Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells

Hyun Taeg Shin, Seok Hee Chung, Jong Soo Lee, Sung Soo Kim, Hyun Dae Shin, Mi Hyeon Jang, Min Chul Shin, Geon Ho Bahn, Eun Kyung Paik, Jae Hyung Park, Chang Ju Kim

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.

Original languageEnglish (US)
Pages (from-to)675-686
Number of pages12
JournalAmerican Journal of Chinese Medicine
Volume31
Issue number5
DOIs
StatePublished - Dec 1 2003

Keywords

  • Corni fructus
  • Hippocampus
  • Hydrogen peroxide
  • N-methyl-D-aspartic acid receptor
  • Rehmanniae radix
  • Shenqi-wan

ASJC Scopus subject areas

  • Complementary and alternative medicine

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    Shin, H. T., Chung, S. H., Lee, J. S., Kim, S. S., Shin, H. D., Jang, M. H., Shin, M. C., Bahn, G. H., Paik, E. K., Park, J. H., & Kim, C. J. (2003). Protective effect of Shenqi-wan against H2O2-induced apoptosis in hippocampal neuronal cells. American Journal of Chinese Medicine, 31(5), 675-686. https://doi.org/10.1142/S0192415X03001454