Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent

Yi Fan Han; Dong Cheng Wu; Xiao Qiu Xiao; Priscilla M.Y. Chen; Wilson Chung; Nelson T.K. Lee; Yuan Ping Pang; Paul R. Carlier

doi:10.1016/S0304-3940(00)01198-8

Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent

Yi Fan Han, Dong Cheng Wu, Xiao Qiu Xiao, Priscilla M.Y. Chen, Wilson Chung, Nelson T.K. Lee, Yuan Ping Pang, Paul R. Carlier

Pharmacology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	95-98
Number of pages	4
Journal	Neuroscience Letters
Volume	288
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(00)01198-8
State	Published - Jul 14 2000

Keywords

Acetylcholinesterase inhibitor
Astrocyte
Bis(7)-tacrine
Ischemia

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(00)01198-8

Cite this

@article{26985f965f04407e9998e4879c10febc,

title = "Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent",

abstract = "The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Acetylcholinesterase inhibitor, Astrocyte, Bis(7)-tacrine, Ischemia",

author = "Han, {Yi Fan} and Wu, {Dong Cheng} and Xiao, {Xiao Qiu} and Chen, {Priscilla M.Y.} and Wilson Chung and Lee, {Nelson T.K.} and Pang, {Yuan Ping} and Carlier, {Paul R.}",

note = "Funding Information: This research was supported by grants from the Research Grants Council (HKUST 6156/97M; 6236/99M) of Hong Kong, and the Biotechnology Research Institute, The Hong Kong University of Science and Technology. Sincere thanks are also given to Dr Albert C.H. Yu and Mr Alberto K.Y. Ng for providing astrocytes and other materials, and to Drs Albert C.H. Yu, Xican Tang and Karl WK. Tsim for advice on the project.",

year = "2000",

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doi = "10.1016/S0304-3940(00)01198-8",

language = "English (US)",

volume = "288",

pages = "95--98",

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AU - Han, Yi Fan

AU - Wu, Dong Cheng

AU - Xiao, Xiao Qiu

AU - Chen, Priscilla M.Y.

AU - Chung, Wilson

AU - Lee, Nelson T.K.

AU - Pang, Yuan Ping

AU - Carlier, Paul R.

N1 - Funding Information: This research was supported by grants from the Research Grants Council (HKUST 6156/97M; 6236/99M) of Hong Kong, and the Biotechnology Research Institute, The Hong Kong University of Science and Technology. Sincere thanks are also given to Dr Albert C.H. Yu and Mr Alberto K.Y. Ng for providing astrocytes and other materials, and to Drs Albert C.H. Yu, Xican Tang and Karl WK. Tsim for advice on the project.

PY - 2000/7/14

Y1 - 2000/7/14

N2 - The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - Acetylcholinesterase inhibitor

KW - Astrocyte

KW - Bis(7)-tacrine

KW - Ischemia

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Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent

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