Abstract
The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-98 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 288 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 14 2000 |
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Keywords
- Acetylcholinesterase inhibitor
- Astrocyte
- Bis(7)-tacrine
- Ischemia
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent. / Han, Yi Fan; Wu, Dong Cheng; Xiao, Xiao Qiu; Chen, Priscilla M Y; Chung, Wilson; Lee, Nelson T K; Pang, Yuan-Ping; Carlier, Paul R.
In: Neuroscience Letters, Vol. 288, No. 2, 14.07.2000, p. 95-98.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent
AU - Han, Yi Fan
AU - Wu, Dong Cheng
AU - Xiao, Xiao Qiu
AU - Chen, Priscilla M Y
AU - Chung, Wilson
AU - Lee, Nelson T K
AU - Pang, Yuan-Ping
AU - Carlier, Paul R.
PY - 2000/7/14
Y1 - 2000/7/14
N2 - The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.
KW - Acetylcholinesterase inhibitor
KW - Astrocyte
KW - Bis(7)-tacrine
KW - Ischemia
UR - http://www.scopus.com/inward/record.url?scp=0034648058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034648058&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(00)01198-8
DO - 10.1016/S0304-3940(00)01198-8
M3 - Article
C2 - 10876069
AN - SCOPUS:0034648058
VL - 288
SP - 95
EP - 98
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -