Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent

Yi Fan Han, Dong Cheng Wu, Xiao Qiu Xiao, Priscilla M.Y. Chen, Wilson Chung, Nelson T.K. Lee, Yuan Ping Pang, Paul R. Carlier

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)95-98
Number of pages4
JournalNeuroscience Letters
Volume288
Issue number2
DOIs
StatePublished - Jul 14 2000

Keywords

  • Acetylcholinesterase inhibitor
  • Astrocyte
  • Bis(7)-tacrine
  • Ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

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