Primary cells and established cell lines join DNA ends with the same efficiency relative to homologous recombination

Hui Zheng, Xiu Bao Chang, John H. Wilson

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative to circularization by homologous recombination between the terminal repeats. The ratios of end joining to homologous recombination were identical in the two types of cells regardless of whether the DNA ends were sticky, blunt, or mismatched. These studies demonstrate that the efficient end joining observed in established cell lines is not a peculiar adaptation to life in culture, but rather reflects a normal aspect of DNA metabolism in mammalian cells. Furthermore, the similar results in primary and established cells support the general validity of using data from studies of cultured cells to formulate models of recombinational processes in primary cells and potentially in multicellular organisms as well.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalPlasmid
Volume22
Issue number2
DOIs
StatePublished - Sep 1989

ASJC Scopus subject areas

  • Molecular Biology

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