Presence of intraepithelial food antigen in patients with active eosinophilic oesophagitis

E. V. Marietta, D. M. Geno, Thomas Christopher Smyrk, A. Becker, J. A. Alexander, Michael Camilleri, Joseph A Murray, David A Katzka

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Background: Although eosinophilic oesophagitis (EoE) is putatively mediated by an abnormal response to food antigen, the oesophagus is considered relatively impermeable to large molecules. Aim: To assess whether food antigens penetrate the oesophageal mucosa in patients with EoE. Methods: Anti-gliadin staining was performed in three groups: active EoE, inactive EoE and EoE patients on a low or gluten free diet. To appraise the specificity of our results, we also performed gliadin staining on six patients without oesophageal disease who were consuming gluten. The groups with EoE on gluten also underwent endoscopic infusion with gluten containing soy sauce and repeat biopsies during the endoscopy. We measured eosinophil density, dilated intercellular spaces (on a 0–4+ scale) and gliadin in oesophageal mucosa by immunofluorescence. Results: Patients with active EoE had significantly greater epithelial density of anti-gliadin staining when compared to inactive EoE (P < 0.0065) and gluten-free patients (P < 0.0008) at baseline and after soy infusion. Gliadin was not detected in non-EoE control patients. The distribution of gliadin was both cytoplasmic and nuclear. There was good correlation of dilated intercellular spaces grade and total gliadin staining intensity (r = 0.577, P = 0.0077). Acute oesophageal perfusion of a commercial gliadin-rich soy sauce did not lead to an increase in gliadin staining in active or inactive EoE. Conclusion: These findings suggest, although do not prove, that antigen penetration in active eosinophilic oesophagitis might be facilitated by impairment of epithelial integrity.

Original languageEnglish (US)
Pages (from-to)427-433
Number of pages7
JournalAlimentary Pharmacology and Therapeutics
Volume45
Issue number3
DOIs
StatePublished - Feb 1 2017

    Fingerprint

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this