Preliminary structural and functional study on a novel gene HSPCSET

Ju Wei, Xiao Jian Sun, Xinyan Wu, Sai Juan Chen, Zhu Chen, Chun Wang, Qiu Hua Huang

Research output: Contribution to journalArticle

Abstract

Objective: To characterize the structural and the functional feature of a novel gene HSPCSET isolated from human CD34 + hematopoietic stem/progenitor cells (HS/PCs). Methods: Bioinformatic technology was used to identify the structural features of the HSPCSET protein and perform the multiple sequence alignment. Yeast-two-hybrid system was used to identify the proteins interacting with the HSPCSET protein. After sequencing, we selected out the positive clones which had clear functions, and carried out β-gal experiment and GST pull down assay to confirm the results. The cellular location of the HSPCSET was checked by immunofluorescence assay. Results: The HSPCSET protein belongs to a SET domain family, which is evolutionarily conserved across species. It implied that HSPCSET may have biologically important function. Using yeast-two-hybrid system, we showed that the protein sequence with SET domain might bind to 13 proteins, which involved in signaling transduction, transcriptional regulation, apoptosis, tumorigenesis, development, etc. And 4 proteins (GADD34, SIVA, DNAJ and PHF1) were confirmed by one-on-one back of the hybrid experiment, β-gal test and GST pull down assay. When GADD34 and HSPCSET were co-transfected, they co-localized in the nucleus, suggesting a strong interaction. Conclusion: The novel gene HSPCSET is likely to have biologically important function. This study provides the basis for further studies of its function in hematopoiesis and tumorigenesis.

Original languageEnglish (US)
Pages (from-to)35-39
Number of pages5
JournalChinese Journal of Medical Genetics
Volume26
Issue number1
DOIs
StatePublished - Feb 10 2009
Externally publishedYes

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Genes
Proteins
Two-Hybrid System Techniques
Hematopoietic Stem Cells
Carcinogenesis
Sequence Alignment
Hematopoiesis
Computational Biology
Fluorescent Antibody Technique
Clone Cells
Apoptosis
Technology

Keywords

  • Hematopoietic stem/progenitor cell
  • HSPCSET gene
  • SET gene family
  • Tumorigenesis

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

Preliminary structural and functional study on a novel gene HSPCSET. / Wei, Ju; Sun, Xiao Jian; Wu, Xinyan; Chen, Sai Juan; Chen, Zhu; Wang, Chun; Huang, Qiu Hua.

In: Chinese Journal of Medical Genetics, Vol. 26, No. 1, 10.02.2009, p. 35-39.

Research output: Contribution to journalArticle

Wei, Ju ; Sun, Xiao Jian ; Wu, Xinyan ; Chen, Sai Juan ; Chen, Zhu ; Wang, Chun ; Huang, Qiu Hua. / Preliminary structural and functional study on a novel gene HSPCSET. In: Chinese Journal of Medical Genetics. 2009 ; Vol. 26, No. 1. pp. 35-39.
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