Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone-binding globulin (TeBG) in human serum

V. Hansson, E. M. Ritzen, S. C. Weddington, W. S. McLean, D. J. Tindall, S. N. Nayfeh, F. S. French

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47 Scopus citations

Abstract

Rabbit serum contains a specific androgen-binding protein (TeBG) with high affinity for 5a-dihydrotestosterone (DHT), 5α-androstane- 3α,17β-diol (Adiol) and testosterone (T). Rabbit TeBG was slightly smaller than human TeBG and had a sedimentation constant of 4-5S (mean 4.4S), a Stokes radius of 43–44 Ågiving a molecular weight of 73,000 and a frictional ratio of 1.6. Measurements of retardation coefficients in polyacrylamide gels of different pore size gave a mean molecular radius of 2.74 nm, indicating a molecular weight of 75,000. Binding to rabbit TeBG was inactivated at pH below 5 or by heating above 50 C for 30 min. Rabbit TeBG was slightly more negatively charged than was human TeBG as demonstrated by ion exchange chromatography. Isoelectric focusing in sucrose gradients or polyacrylamide gels indicated a pi of 5.2 for rabbit TeBG and 5.5 for human TeBG. At 0 C the dissociation of DHT from rabbit TeBG (t1/2 = 5.2 min) was much faster than the dissociation of DHT from human TeBG (t1/2 = 67 min). The steroid specificity of rabbit TeBG was similar to that of human TeBG: DHT > A-diol > T >>>progesterone, androst-4-ene-3,17-dione and cortisol. The equilibrium constant (Ka) for binding of DHT to TeBG at 0 C was 1.9 Å– 109 M-1. Binding affinity of rabbit TeBG for DHT was markedly lowered by increase in temperature (Ka 37 C = 1.2 Å– 108 M-1) As in human, higher concentrations of rabbit TeBG were found in adult females than in males (p < 0.01). In immature rabbits, TeBG concentrations were much higher than in adult.

Original languageEnglish (US)
Pages (from-to)690-700
Number of pages11
JournalEndocrinology
Volume95
Issue number3
DOIs
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Endocrinology

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    Hansson, V., Ritzen, E. M., Weddington, S. C., McLean, W. S., Tindall, D. J., Nayfeh, S. N., & French, F. S. (1974). Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone-binding globulin (TeBG) in human serum. Endocrinology, 95(3), 690-700. https://doi.org/10.1210/endo-95-3-690