Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone binding globulin (TeBG) in human serum

V. Hansson, E. M. Ritzen, S. C. Weddington, W. S. McLean, D. J. Tindall, S. N. Nayfeh, F. S. French

Research output: Contribution to journalArticle

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Abstract

Rabbit serum contains a specific androgen binding protein (TeBG) with high affinity for 5α dihydrotestosterone (DHT), 5α androstane 3α,17b diol (Adiol) and testosterone (T). Rabbit TeBG was slightly smaller than human TeBG and had a sedimentation constant of 4-5S (mean 4.4S), a Stokes radius of 43-44 Å giving a mol wt of 73,000 and a frictional ratio of 1.6. Measurements of retardation coefficients in polyacrylamide gels of different pore size gave a mean molecular radius of 2.74 nm, indicating a mol wt of 75,000. Binding to rabbit TeBG was inactivated at pH below 5 or by heating above 50 C for 30 min. Rabbit TeBG was slightly more negatively charged than was human TeBG as demonstrated by ion exchange chromatography. Isoelectric focusing in sucrose gradients or polyacrylamide gels indicated a pI of 5.2 for rabbit TeBG and 5.5 for human TeBG. At 0 C the dissociation of DHT from rabbit TeBG (t1/2 = 5.2 min) was much faster than the dissociation of DHT from human TeBG (t1/2 = 67 min). The steroid specificity of rabbit TeBG was similar to that of human TeBG: DHT > Adiol > T >>> progesterone,androst 4 ene 3,17 dione and cortisol. The equilibrium constant (Ka) for binding of DHT to TeBG at 0 C was 1.9 x 10 9 M -1. Binding affinity of rabbit TeBG for DHT was markedly lowered by increase in temperature (Ka 37 C = 1.2 x 10 8 M -1). As in humans, higher concentrations of rabbit TeBG were found in adult females than in males (p < 0.01). In immature rabbits, TeBG concentrations were much higher than in adult rabbits. (26 references).

Original languageEnglish (US)
Pages (from-to)690-700
Number of pages11
JournalEndocrinology
Volume95
Issue number3
StatePublished - 1974
Externally publishedYes

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Androgen-Binding Protein
Globulins
Testosterone
Rabbits
Serum
Dihydrotestosterone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hansson, V., Ritzen, E. M., Weddington, S. C., McLean, W. S., Tindall, D. J., Nayfeh, S. N., & French, F. S. (1974). Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone binding globulin (TeBG) in human serum. Endocrinology, 95(3), 690-700.

Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone binding globulin (TeBG) in human serum. / Hansson, V.; Ritzen, E. M.; Weddington, S. C.; McLean, W. S.; Tindall, D. J.; Nayfeh, S. N.; French, F. S.

In: Endocrinology, Vol. 95, No. 3, 1974, p. 690-700.

Research output: Contribution to journalArticle

Hansson, V, Ritzen, EM, Weddington, SC, McLean, WS, Tindall, DJ, Nayfeh, SN & French, FS 1974, 'Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone binding globulin (TeBG) in human serum', Endocrinology, vol. 95, no. 3, pp. 690-700.
Hansson, V. ; Ritzen, E. M. ; Weddington, S. C. ; McLean, W. S. ; Tindall, D. J. ; Nayfeh, S. N. ; French, F. S. / Preliminary characterization of a binding protein for androgen in rabbit serum. Comparison with the testosterone binding globulin (TeBG) in human serum. In: Endocrinology. 1974 ; Vol. 95, No. 3. pp. 690-700.
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AU - Hansson, V.

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AU - McLean, W. S.

AU - Tindall, D. J.

AU - Nayfeh, S. N.

AU - French, F. S.

PY - 1974

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