TY - JOUR
T1 - Pregnancy-associated plasma protein-A expression in human breast cancer
AU - Mansfield, Aaron S.
AU - Visscher, Daniel W.
AU - Hart, Steven N.
AU - Wang, Chen
AU - Goetz, Matthew P.
AU - Oxvig, Claus
AU - Conover, Cheryl A.
N1 - Funding Information:
Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under award number P50CA 116201 , and an unrestricted research grant from Ansh Labs (to CAC). The content is solely the responsibility of the authors and does not necessarily represent the official view of the National Institutes of Health. The funding sources had no involvement in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication.
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Objective: Pregnancy-associated plasma protein-A (PAPP-A) is a zinc metalloproteinase in the insulin-like growth factor system that is expressed by tissues outside of pregnancy and involved in normal and dysregulated growth. PAPP-A has been implicated in several cancers. However, studies of PAPP-A expression in breast cancer are limited. In this study, we assessed PAPP-A expression in different subtypes of human malignant breast cancer.Design Formalin-fixed paraffin-embedded tumor samples from 46 female patients with invasive breast cancer were divided into five defined groups [using markers for HER2, estrogen receptor, progesterone receptor, proliferation] that roughly correlate with molecularly defined subtypes (luminal A, luminal B, luminal/HER2. +, HER2. +, triple negative). These samples were analyzed for PAPP-A expression by immunohistochemistry. Results: PAPP-A staining in tumor tissue was detected in 45 of 46 specimens. There were significantly greater extent and intensity of PAPP-A expression in luminal B specimens with high proliferation index than luminal A specimens (. P=. 0.01). However, there were no differences between specimens positive or negative for HER2 (. P=. 0.14) or positive and negative for estrogen receptor (. P=. 0.31). Conclusion: PAPP-A was detected in almost all breast cancer specimens and a more intense and greater extent of its expression was associated with luminal B specimens compared to luminal A specimens. The role of PAPP-A in breast cancer prognosis, and possibly therapeutics, warrants further investigation.
AB - Objective: Pregnancy-associated plasma protein-A (PAPP-A) is a zinc metalloproteinase in the insulin-like growth factor system that is expressed by tissues outside of pregnancy and involved in normal and dysregulated growth. PAPP-A has been implicated in several cancers. However, studies of PAPP-A expression in breast cancer are limited. In this study, we assessed PAPP-A expression in different subtypes of human malignant breast cancer.Design Formalin-fixed paraffin-embedded tumor samples from 46 female patients with invasive breast cancer were divided into five defined groups [using markers for HER2, estrogen receptor, progesterone receptor, proliferation] that roughly correlate with molecularly defined subtypes (luminal A, luminal B, luminal/HER2. +, HER2. +, triple negative). These samples were analyzed for PAPP-A expression by immunohistochemistry. Results: PAPP-A staining in tumor tissue was detected in 45 of 46 specimens. There were significantly greater extent and intensity of PAPP-A expression in luminal B specimens with high proliferation index than luminal A specimens (. P=. 0.01). However, there were no differences between specimens positive or negative for HER2 (. P=. 0.14) or positive and negative for estrogen receptor (. P=. 0.31). Conclusion: PAPP-A was detected in almost all breast cancer specimens and a more intense and greater extent of its expression was associated with luminal B specimens compared to luminal A specimens. The role of PAPP-A in breast cancer prognosis, and possibly therapeutics, warrants further investigation.
KW - Breast cancer
KW - Immunohistochemistry
KW - Pregnancy-associated plasma protein-A
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U2 - 10.1016/j.ghir.2014.10.007
DO - 10.1016/j.ghir.2014.10.007
M3 - Article
C2 - 25468445
AN - SCOPUS:84919474294
SN - 1096-6374
VL - 24
SP - 264
EP - 267
JO - Growth Hormone and IGF Research
JF - Growth Hormone and IGF Research
IS - 6
ER -