Polyploidy associated with oxidative injury attenuates proliferative potential of cells

G. R. Gorla, Harmeet M Malhi, S. Gupta

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Polyploid cells are encountered ubiquitously but the biological significance of polyploidy is unclear. In view of their extensive capacity for regeneration, hepatocytes offer excellent systems for analyzing growth control mechanisms. We isolated hepatocytes from adult rats with and without two-third partial hepatectomy, which induces hepatic polyploidy. Polyploid hepatocytes showed evidence for oxidative injury with antioxidant depletion, lipid peroxidation and 8-hydroxy-adducts of guanine in nuclear DNA. Liver repopulation assays in intact animals showed markedly decreased replication capacity in polyploid hepatocytes. Recapitulation of polyploidy in cultured hepatocytes established that mitogenic stimulation in the presence of oxidative DNA injury was capable of inducing polyploidy. The findings provide novel frameworks in the context of polyploidy for understanding tissue development, regeneration and oncogenesis.

Original languageEnglish (US)
Pages (from-to)2943-2951
Number of pages9
JournalJournal of Cell Science
Volume114
Issue number16
StatePublished - 2001
Externally publishedYes

Fingerprint

Polyploidy
Wounds and Injuries
Hepatocytes
Regeneration
Liver
Guanine
Hepatectomy
Lipid Peroxidation
DNA Damage
Carcinogenesis
Antioxidants
DNA
Growth

Keywords

  • Growth factor
  • Hepatocyte
  • Liver
  • Ploidy
  • Regeneration

ASJC Scopus subject areas

  • Cell Biology

Cite this

Polyploidy associated with oxidative injury attenuates proliferative potential of cells. / Gorla, G. R.; Malhi, Harmeet M; Gupta, S.

In: Journal of Cell Science, Vol. 114, No. 16, 2001, p. 2943-2951.

Research output: Contribution to journalArticle

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