Pituitary expression of protein kinase C isotypes during early development

Andrew I. Korytko, Alan P Fields, Lisa A. Allshouse, Leona Cuttler

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Protein kinase C (PKC) is a critical regulator of signal transduction and cell function in many tissues, including pituitary. Although PKC influences pituitary hormone secretion in adults, its role in determining characteristic perinatal patterns of hormone secretion and synthesis is not known, and the expression of major PKC isotypes in perinatal pituitary is poorly defined. We therefore determined the developmental, cell-specific expression of the major PKC isotypes, using Western analysis and double label immunohistochemistry, in pituitaries of perinatal and mature rats. Expression of specific PKC isotypes was strikingly age-dependent. Pituitary expression of PKC α was particularly high in neonates and declined significantly with age, with levels in adult rats approximately half those of neonates as assessed by Western analysis. Similarly, immunohistochemistry indicated that PKC α was less abundant in adult than in neonatal pituitaries; the most intensely staining cells of both age groups were identified as somatotrophs and gonadotrophs, In contrast to PKC α, pituitary expression of PKC ε increased approximately two-fold with advancing age as assessed by Western analysis; this age-dependent pattern was confirmed by immunohistochemistry. Perinatal pituitaries expressed PKC ε in some somatotrophs and in all gonadotrophs, whereas PKC ε expression was limited to gonadotrophs in the mature pituitary. Pituitary expression of PKC β@?, δ, and ζ did not differ with age, and PKC γ was not detected in pituitaries of any age group. These results indicate that expression of PKC isotypes within the pituitary is developmentally regulated in a cell-specific and isotype-specific manner, and are consistent with the concept that PKC contributes to the regulation of pituitary function during early development.

Original languageEnglish (US)
Pages (from-to)569-576
Number of pages8
JournalJournal of Neuroendocrinology
Volume10
Issue number8
DOIs
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Protein Kinase C
Gonadotrophs
Somatotrophs
Immunohistochemistry
Age Groups
Newborn Infant
Pituitary Hormones
Signal Transduction
Hormones

Keywords

  • Isotypes
  • Newborn
  • Ontogeny
  • Pituitary
  • Protein kinase C

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Pituitary expression of protein kinase C isotypes during early development. / Korytko, Andrew I.; Fields, Alan P; Allshouse, Lisa A.; Cuttler, Leona.

In: Journal of Neuroendocrinology, Vol. 10, No. 8, 08.1998, p. 569-576.

Research output: Contribution to journalArticle

Korytko, Andrew I. ; Fields, Alan P ; Allshouse, Lisa A. ; Cuttler, Leona. / Pituitary expression of protein kinase C isotypes during early development. In: Journal of Neuroendocrinology. 1998 ; Vol. 10, No. 8. pp. 569-576.
@article{11b8cf15b8d14315beb4423ce6866cf2,
title = "Pituitary expression of protein kinase C isotypes during early development",
abstract = "Protein kinase C (PKC) is a critical regulator of signal transduction and cell function in many tissues, including pituitary. Although PKC influences pituitary hormone secretion in adults, its role in determining characteristic perinatal patterns of hormone secretion and synthesis is not known, and the expression of major PKC isotypes in perinatal pituitary is poorly defined. We therefore determined the developmental, cell-specific expression of the major PKC isotypes, using Western analysis and double label immunohistochemistry, in pituitaries of perinatal and mature rats. Expression of specific PKC isotypes was strikingly age-dependent. Pituitary expression of PKC α was particularly high in neonates and declined significantly with age, with levels in adult rats approximately half those of neonates as assessed by Western analysis. Similarly, immunohistochemistry indicated that PKC α was less abundant in adult than in neonatal pituitaries; the most intensely staining cells of both age groups were identified as somatotrophs and gonadotrophs, In contrast to PKC α, pituitary expression of PKC ε increased approximately two-fold with advancing age as assessed by Western analysis; this age-dependent pattern was confirmed by immunohistochemistry. Perinatal pituitaries expressed PKC ε in some somatotrophs and in all gonadotrophs, whereas PKC ε expression was limited to gonadotrophs in the mature pituitary. Pituitary expression of PKC β@?, δ, and ζ did not differ with age, and PKC γ was not detected in pituitaries of any age group. These results indicate that expression of PKC isotypes within the pituitary is developmentally regulated in a cell-specific and isotype-specific manner, and are consistent with the concept that PKC contributes to the regulation of pituitary function during early development.",
keywords = "Isotypes, Newborn, Ontogeny, Pituitary, Protein kinase C",
author = "Korytko, {Andrew I.} and Fields, {Alan P} and Allshouse, {Lisa A.} and Leona Cuttler",
year = "1998",
month = "8",
doi = "10.1046/j.1365-2826.1998.00201.x",
language = "English (US)",
volume = "10",
pages = "569--576",
journal = "Journal of Neuroendocrinology",
issn = "0953-8194",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Pituitary expression of protein kinase C isotypes during early development

AU - Korytko, Andrew I.

AU - Fields, Alan P

AU - Allshouse, Lisa A.

AU - Cuttler, Leona

PY - 1998/8

Y1 - 1998/8

N2 - Protein kinase C (PKC) is a critical regulator of signal transduction and cell function in many tissues, including pituitary. Although PKC influences pituitary hormone secretion in adults, its role in determining characteristic perinatal patterns of hormone secretion and synthesis is not known, and the expression of major PKC isotypes in perinatal pituitary is poorly defined. We therefore determined the developmental, cell-specific expression of the major PKC isotypes, using Western analysis and double label immunohistochemistry, in pituitaries of perinatal and mature rats. Expression of specific PKC isotypes was strikingly age-dependent. Pituitary expression of PKC α was particularly high in neonates and declined significantly with age, with levels in adult rats approximately half those of neonates as assessed by Western analysis. Similarly, immunohistochemistry indicated that PKC α was less abundant in adult than in neonatal pituitaries; the most intensely staining cells of both age groups were identified as somatotrophs and gonadotrophs, In contrast to PKC α, pituitary expression of PKC ε increased approximately two-fold with advancing age as assessed by Western analysis; this age-dependent pattern was confirmed by immunohistochemistry. Perinatal pituitaries expressed PKC ε in some somatotrophs and in all gonadotrophs, whereas PKC ε expression was limited to gonadotrophs in the mature pituitary. Pituitary expression of PKC β@?, δ, and ζ did not differ with age, and PKC γ was not detected in pituitaries of any age group. These results indicate that expression of PKC isotypes within the pituitary is developmentally regulated in a cell-specific and isotype-specific manner, and are consistent with the concept that PKC contributes to the regulation of pituitary function during early development.

AB - Protein kinase C (PKC) is a critical regulator of signal transduction and cell function in many tissues, including pituitary. Although PKC influences pituitary hormone secretion in adults, its role in determining characteristic perinatal patterns of hormone secretion and synthesis is not known, and the expression of major PKC isotypes in perinatal pituitary is poorly defined. We therefore determined the developmental, cell-specific expression of the major PKC isotypes, using Western analysis and double label immunohistochemistry, in pituitaries of perinatal and mature rats. Expression of specific PKC isotypes was strikingly age-dependent. Pituitary expression of PKC α was particularly high in neonates and declined significantly with age, with levels in adult rats approximately half those of neonates as assessed by Western analysis. Similarly, immunohistochemistry indicated that PKC α was less abundant in adult than in neonatal pituitaries; the most intensely staining cells of both age groups were identified as somatotrophs and gonadotrophs, In contrast to PKC α, pituitary expression of PKC ε increased approximately two-fold with advancing age as assessed by Western analysis; this age-dependent pattern was confirmed by immunohistochemistry. Perinatal pituitaries expressed PKC ε in some somatotrophs and in all gonadotrophs, whereas PKC ε expression was limited to gonadotrophs in the mature pituitary. Pituitary expression of PKC β@?, δ, and ζ did not differ with age, and PKC γ was not detected in pituitaries of any age group. These results indicate that expression of PKC isotypes within the pituitary is developmentally regulated in a cell-specific and isotype-specific manner, and are consistent with the concept that PKC contributes to the regulation of pituitary function during early development.

KW - Isotypes

KW - Newborn

KW - Ontogeny

KW - Pituitary

KW - Protein kinase C

UR - http://www.scopus.com/inward/record.url?scp=0031849340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031849340&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2826.1998.00201.x

DO - 10.1046/j.1365-2826.1998.00201.x

M3 - Article

VL - 10

SP - 569

EP - 576

JO - Journal of Neuroendocrinology

JF - Journal of Neuroendocrinology

SN - 0953-8194

IS - 8

ER -