Photoacoustic molecular imaging

William L. Kiser, Daniel Reinecke, Timothy R DeGrado, Sibaprasad Bhattacharyya, Robert A. Kruger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

It is well documented that photoacoustic imaging has the capability to differentiate tissue based on the spectral characteristics of tissue in the optical regime. The imaging depth in tissue exceeds standard optical imaging techniques, and systems can be designed to achieve excellent spatial resolution. A natural extension of imaging the intrinsic optical contrast of tissue is to demonstrate the ability of photoacoustic imaging to detect contrast agents based on optically absorbing dyes that exhibit well defined absorption peaks in the infrared. The ultimate goal of this project is to implement molecular imaging, in which Herceptin™, a monoclonal antibody that is used as a therapeutic agent in breast cancer patients that over express the HER2 gene, is labeled with an IR absorbing dye, and the resulting in vivo biodistribution is mapped using multi-spectral, infrared stimulation and subsequent photoacoustic detection. To lay the groundwork for this goal and establish system sensitivity, images were collected in tissue mimicking phantoms to determine maximum detection depth and minimum detectable concentration of Indocyanine Green (ICG), a common IR absorbing dye, for a single angle photoacoustic acquisition. A breast mimicking phantom was constructed and spectra were also collected for hemoglobin and methanol. An imaging schema was developed that made it possible to separate the ICG from the other tissue mimicking components in a multiple component phantom. We present the results of these experiments and define the path forward for the detection of dye labeled Herceptin™ in cell cultures and mice models.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6437
DOIs
StatePublished - 2007
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - San Jose, CA, United States
Duration: Jan 21 2007Jan 24 2007

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
CountryUnited States
CitySan Jose, CA
Period1/21/071/24/07

Fingerprint

Molecular imaging
Photoacoustic effect
Tissue
Imaging techniques
Dyes
Infrared radiation
Monoclonal antibodies
Hemoglobin
Cell culture
Imaging systems
Methanol
Genes

Keywords

  • Herceptin
  • Molecular imaging
  • Photoacoustic imaging
  • Targeted contrast agent

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kiser, W. L., Reinecke, D., DeGrado, T. R., Bhattacharyya, S., & Kruger, R. A. (2007). Photoacoustic molecular imaging. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6437). [643721] https://doi.org/10.1117/12.705136

Photoacoustic molecular imaging. / Kiser, William L.; Reinecke, Daniel; DeGrado, Timothy R; Bhattacharyya, Sibaprasad; Kruger, Robert A.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6437 2007. 643721.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kiser, WL, Reinecke, D, DeGrado, TR, Bhattacharyya, S & Kruger, RA 2007, Photoacoustic molecular imaging. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6437, 643721, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.705136
Kiser WL, Reinecke D, DeGrado TR, Bhattacharyya S, Kruger RA. Photoacoustic molecular imaging. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6437. 2007. 643721 https://doi.org/10.1117/12.705136
Kiser, William L. ; Reinecke, Daniel ; DeGrado, Timothy R ; Bhattacharyya, Sibaprasad ; Kruger, Robert A. / Photoacoustic molecular imaging. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6437 2007.
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