Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly

Bin Kang; Mintie Pu; Gangqing Hu; Weihong Wen; Zigang Dong; Keji Zhao; Bruce Stillman; Zhiguo Zhang

doi:10.1101/gad.2055511

Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly

Bin Kang, Mintie Pu, Gangqing Hu, Weihong Wen, Zigang Dong, Keji Zhao, Bruce Stillman, Zhiguo Zhang

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction. Here, we show that phosphorylation of histone H4 Ser 47 (H4S47ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4. These results reveal a mechanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3.

Original language	English (US)
Pages (from-to)	1359-1364
Number of pages	6
Journal	Genes and Development
Volume	25
Issue number	13
DOIs	https://doi.org/10.1101/gad.2055511
State	Published - Jul 1 2011

Keywords

CAF-1
HIRA
Histone H3.3
Histone variant
Nucleosome assembly
PAK2

ASJC Scopus subject areas

General Medicine

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10.1101/gad.2055511

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title = "Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly",

abstract = "Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction. Here, we show that phosphorylation of histone H4 Ser 47 (H4S47ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4. These results reveal a mechanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3.",

keywords = "CAF-1, HIRA, Histone H3.3, Histone variant, Nucleosome assembly, PAK2",

author = "Bin Kang and Mintie Pu and Gangqing Hu and Weihong Wen and Zigang Dong and Keji Zhao and Bruce Stillman and Zhiguo Zhang",

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AU - Kang, Bin

AU - Pu, Mintie

AU - Hu, Gangqing

AU - Wen, Weihong

AU - Dong, Zigang

AU - Zhao, Keji

AU - Stillman, Bruce

AU - Zhang, Zhiguo

PY - 2011/7/1

Y1 - 2011/7/1

N2 - Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction. Here, we show that phosphorylation of histone H4 Ser 47 (H4S47ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4. These results reveal a mechanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3.

AB - Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction. Here, we show that phosphorylation of histone H4 Ser 47 (H4S47ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4. These results reveal a mechanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3.

KW - CAF-1

KW - HIRA

KW - Histone H3.3

KW - Histone variant

KW - Nucleosome assembly

KW - PAK2

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SN - 0890-9369

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JO - Genes and Development

JF - Genes and Development

IS - 13

ER -

Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly

Abstract

Keywords

ASJC Scopus subject areas

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