TY - JOUR
T1 - Pattern of p53 gene mutations in breast cancers of women of the Midwestern United States
AU - Sommer, Steve S.
AU - Cunningham, Julie
AU - Mcgovern, Renee M.
AU - Saitoh, Soh
AU - Schroeder, Jennifer J.
AU - Wold, Lester E.
AU - Kovach, John S.
N1 - Funding Information:
Supported in part by Public Health Service grant CA-15083 from the National Cancer Institute, National Institutes of Health, Department of Health and Human Services.
Funding Information:
Dedicated in memory of Poldi Hirsch. We gratefully acknowledge the long-term support of cancer research at the Mayo Foundation by Helen C. Levitt, Minneapolis, Minn., and Boca Raton, Fla. We thank Ms. Diane Fitch for her expert assistance in facilitating tissue procurement.
PY - 1992/2/19
Y1 - 1992/2/19
N2 - Background: Mutation in the p53 gene is the most common genetic lesion in human cancers. The pattern of muta-tion in the p53 gene differs among can-cers and may be a useful epidemiological tool for identification of factors contributing to carcinogenesis. Pur-pose: Our purpose was to determine if the pattern of p53 mutation in breast carcinomas in our population of women residing in the midwestern region of the United States is similar to the pattern of p53 mutation in breast cancers in patients from other regions of the United States and Europe and in other epithelial tumors. Methods: With a technique we recently developed for the analysis of p53 mutations in genomic DNA from tumor cell clusters in touch preparations of solid tumors, we sequenced exons 5-9 and adjacent splice junctions of the gene in 44 breast cancers. Cells from each tumor were also stained with three monoclonal an-tibodies which recognize different epitopes of the p53 protein. Results: We detected p53 mutations in 14 (32.6%) of 44 breast carcinomas. Only half of the mutations were missense changes. The other half included five microdeletions (three producing frame-shifts), one single-base substitution generating a stop codon, and one single-base substitution generating a splice junction abnormality. Nuclear expression of p53 antigen was present in eight of 44 cancers, including six with hemizygous missense mutations in the p53 gene. Conclusions: The pattern of p53 mutations in our breast cancer population differs from that reported in breast cancer populations by other investigators in which most p53 muta-tions were missense. Among 14 muta-tions in our population, at least five drastically altered the structure of p53, suggesting that a recessive mechanism of inactivation of the p53 gene may be more common than in other popula-tions. Implications: Differences in the pattern of p53 mutation in breast can-cers in Midwestern women and in breast cancers in other populations may reflect selection bias or small sample sizes currently available. How-ever, our data are compatible with the possibility that an endogenous or exogenous factor influences p53 car-cinogenesis in some women with breast cancer in the Midwest to a greater ex-tent than in other regions of the United States and Europe. [J Natl Cancer Inst 84: 246- 252, 1992]
AB - Background: Mutation in the p53 gene is the most common genetic lesion in human cancers. The pattern of muta-tion in the p53 gene differs among can-cers and may be a useful epidemiological tool for identification of factors contributing to carcinogenesis. Pur-pose: Our purpose was to determine if the pattern of p53 mutation in breast carcinomas in our population of women residing in the midwestern region of the United States is similar to the pattern of p53 mutation in breast cancers in patients from other regions of the United States and Europe and in other epithelial tumors. Methods: With a technique we recently developed for the analysis of p53 mutations in genomic DNA from tumor cell clusters in touch preparations of solid tumors, we sequenced exons 5-9 and adjacent splice junctions of the gene in 44 breast cancers. Cells from each tumor were also stained with three monoclonal an-tibodies which recognize different epitopes of the p53 protein. Results: We detected p53 mutations in 14 (32.6%) of 44 breast carcinomas. Only half of the mutations were missense changes. The other half included five microdeletions (three producing frame-shifts), one single-base substitution generating a stop codon, and one single-base substitution generating a splice junction abnormality. Nuclear expression of p53 antigen was present in eight of 44 cancers, including six with hemizygous missense mutations in the p53 gene. Conclusions: The pattern of p53 mutations in our breast cancer population differs from that reported in breast cancer populations by other investigators in which most p53 muta-tions were missense. Among 14 muta-tions in our population, at least five drastically altered the structure of p53, suggesting that a recessive mechanism of inactivation of the p53 gene may be more common than in other popula-tions. Implications: Differences in the pattern of p53 mutation in breast can-cers in Midwestern women and in breast cancers in other populations may reflect selection bias or small sample sizes currently available. How-ever, our data are compatible with the possibility that an endogenous or exogenous factor influences p53 car-cinogenesis in some women with breast cancer in the Midwest to a greater ex-tent than in other regions of the United States and Europe. [J Natl Cancer Inst 84: 246- 252, 1992]
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U2 - 10.1093/jnci/84.4.246
DO - 10.1093/jnci/84.4.246
M3 - Article
C2 - 1734086
AN - SCOPUS:0026554642
SN - 0027-8874
VL - 84
SP - 246
EP - 252
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 4
ER -