Pathological considerations of laser-tissue interactions: Light microscopic assessment of thermal damage of skin

Thomas J. Flotte, Margaret Goetschkes

Research output: Contribution to journalConference article

Abstract

A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.

Original languageEnglish (US)
Pages (from-to)278-283
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1643
DOIs
StatePublished - Jun 1 1992
EventLaser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992 - Los Angeles, United States
Duration: Jan 19 1992Jan 24 1992

Fingerprint

Laser tissue interaction
Skin
Coloring Agents
Damage
Laser
damage
Lasers
Interaction
Thermal effects
lasers
interactions
Fixatives
Acids
temperature effects
Thermal Effects
acids
Hot Temperature
matrices
Lower bound

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

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title = "Pathological considerations of laser-tissue interactions: Light microscopic assessment of thermal damage of skin",
abstract = "A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.",
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AB - A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.

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