Pathological considerations of laser-tissue interactions: Light microscopic assessment of thermal damage of skin

Thomas J. Flotte; Margaret Goetschkes

doi:10.1117/12.137355

Pathological considerations of laser-tissue interactions: Light microscopic assessment of thermal damage of skin

Thomas J. Flotte, Margaret Goetschkes

Laboratory Medicine and Pathology

Research output: Contribution to journal › Conference article › peer-review

Abstract

A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.

Original language	English (US)
Pages (from-to)	278-283
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1643
DOIs	https://doi.org/10.1117/12.137355
State	Published - Jun 1 1992
Event	Laser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992 - Los Angeles, United States Duration: Jan 19 1992 → Jan 24 1992

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.137355

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title = "Pathological considerations of laser-tissue interactions: Light microscopic assessment of thermal damage of skin",

abstract = "A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.",

author = "Flotte, {Thomas J.} and Margaret Goetschkes",

note = "Publisher Copyright: {\textcopyright} 1992 SPIE. All rights reserved.; Laser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992 ; Conference date: 19-01-1992 Through 24-01-1992",

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T2 - Laser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992

AU - Flotte, Thomas J.

AU - Goetschkes, Margaret

PY - 1992/6/1

Y1 - 1992/6/1

N2 - A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.

AB - A variety of fixatives and stains were examined for the ability to differentially stain the extracellular matrix components of thermal damage to the skin in an attempt to provide methods for examining the extent of thermal effects. This information is important in comparing different lasers and laser parameters. Four zones of thermal damage were identified including char and three zones of less extensive damage. The lower bounds of the damage with steady state conditions for these zones were 64-66 °C, 80-85 °C, and > 100 °C. The best choices based on this study include the following: Fixative: Bouin's, overall stain: H & E, inner zone stain: Pinkus' acid orcein giemsa, middle zone stain: Movat's pentachrome, and outer zone stain: The modified elastic stain presented in the appendix of this paper.

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JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

Y2 - 19 January 1992 through 24 January 1992

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Pathological considerations of laser-tissue interactions: Light microscopic assessment of thermal damage of skin

Abstract

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