Pathogen-Derived Effectors Trigger Protective Immunity via Activation of the Rac2 Enzyme and the IMD or Rip Kinase Signaling Pathway

Laurent Boyer, Lorin Magoc, Stephanie Dejardin, Michael Cappillino, Nicholas Paquette, Charlotte Hinault, Guillaume M. Charriere, Wai Kee Eddie Ip, Shannon Fracchia, Elizabeth Hennessy, Deniz Erturk-Hasdemir, Jean Marc Reichhart, Neal Silverman, Adam Lacy-Hulbert, Lynda M. Stuart

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Although infections with virulent pathogens often induce a strong inflammatory reaction, what drives the increased immune response to pathogens compared to nonpathogenic microbes is poorly understood. One possibility is that the immune system senses the level of threat from a microorganism and augments the response accordingly. Here, focusing on cytotoxic necrotizing factor 1 (CNF1), an Escherichia coli-derived effector molecule, we showed the host indirectly sensed the pathogen by monitoring for the effector that modified RhoGTPases. CNF1 modified Rac2, which then interacted with the innate immune adaptors IMD and Rip1-Rip2 in flies and mammalian cells, respectively, to drive an immune response. This response was protective and increased the ability of the host to restrict pathogen growth, thus defining a mechanism of effector-triggered immunity that contributes to how metazoans defend against microbes with pathogenic potential.

Original languageEnglish (US)
Pages (from-to)536-549
Number of pages14
JournalImmunity
Volume35
Issue number4
DOIs
StatePublished - Oct 28 2011
Externally publishedYes

Fingerprint

Enzyme Activation
Immunity
Phosphotransferases
Diptera
Immune System
Escherichia coli
Growth
Infection
cytotoxic necrotizing factor type 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Boyer, L., Magoc, L., Dejardin, S., Cappillino, M., Paquette, N., Hinault, C., ... Stuart, L. M. (2011). Pathogen-Derived Effectors Trigger Protective Immunity via Activation of the Rac2 Enzyme and the IMD or Rip Kinase Signaling Pathway. Immunity, 35(4), 536-549. https://doi.org/10.1016/j.immuni.2011.08.015

Pathogen-Derived Effectors Trigger Protective Immunity via Activation of the Rac2 Enzyme and the IMD or Rip Kinase Signaling Pathway. / Boyer, Laurent; Magoc, Lorin; Dejardin, Stephanie; Cappillino, Michael; Paquette, Nicholas; Hinault, Charlotte; Charriere, Guillaume M.; Ip, Wai Kee Eddie; Fracchia, Shannon; Hennessy, Elizabeth; Erturk-Hasdemir, Deniz; Reichhart, Jean Marc; Silverman, Neal; Lacy-Hulbert, Adam; Stuart, Lynda M.

In: Immunity, Vol. 35, No. 4, 28.10.2011, p. 536-549.

Research output: Contribution to journalArticle

Boyer, L, Magoc, L, Dejardin, S, Cappillino, M, Paquette, N, Hinault, C, Charriere, GM, Ip, WKE, Fracchia, S, Hennessy, E, Erturk-Hasdemir, D, Reichhart, JM, Silverman, N, Lacy-Hulbert, A & Stuart, LM 2011, 'Pathogen-Derived Effectors Trigger Protective Immunity via Activation of the Rac2 Enzyme and the IMD or Rip Kinase Signaling Pathway', Immunity, vol. 35, no. 4, pp. 536-549. https://doi.org/10.1016/j.immuni.2011.08.015
Boyer, Laurent ; Magoc, Lorin ; Dejardin, Stephanie ; Cappillino, Michael ; Paquette, Nicholas ; Hinault, Charlotte ; Charriere, Guillaume M. ; Ip, Wai Kee Eddie ; Fracchia, Shannon ; Hennessy, Elizabeth ; Erturk-Hasdemir, Deniz ; Reichhart, Jean Marc ; Silverman, Neal ; Lacy-Hulbert, Adam ; Stuart, Lynda M. / Pathogen-Derived Effectors Trigger Protective Immunity via Activation of the Rac2 Enzyme and the IMD or Rip Kinase Signaling Pathway. In: Immunity. 2011 ; Vol. 35, No. 4. pp. 536-549.
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