Participation of Vβ13+ and Vβ1+ T cells in transfer thyroiditis after activation of mouse thyroglobulin-primed T cells by superantigen staphylococcal enterotoxin A

Qiang Wan, Masako Kita, Jeffrey C. Flynn, John C. Panos, Reinhard W. Motte, Terry F. Davies, Alvaro A. Giraldo, Chella S. David, Yi Chi M. Kong

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Murine experimental autoimmune thyroiditis (EAT) is a T-cell-mediated disease, but the T cell receptor (TCR) Vβ gene usage in pathogenesis has not been well delineated. One approach is to utilize bacterial superantigens, such as staphylococcal enterotoxin (SE) A and B, to stimulate known sets of TCR Vβ families in mouse thyroglobulin (mTg)-primed cells for thyroiditis transfer. Our previous use of SEB to activate mTg-primed cells led to no thyroiditis transfer, despite a major increase in Vβ8+ T cells. Unlike SEB, SEA activation did transfer thyroiditis. To determine which thyroiditogenic Vβ+ T cells were involved, SEA-activated T cells have now been analyzed. After repeated SEA activation in vitro, both mTg-reactive and thyroiditogenic cells persisted. FACS analysis indicated that most Vβ13+ cells were "large" cells (IL-2R+) and expressed the activation marker, transferrin receptor (CD71). RT-PCR analysis also showed the presence of both Vβ13+ and SEA-reactive Vβ1+ cells. Since our previous analyses by RT-PCR of the thyroid infiltrate after either induction or adoptive transfer have implicated both Vβ13+ and Vβ1+ cells, their activation by SEA to transfer thyroiditis further supports their role.

Original languageEnglish (US)
Pages (from-to)149-157
Number of pages9
JournalCellular Immunology
Volume213
Issue number2
DOIs
StatePublished - Nov 1 2001

Keywords

  • Autoimmunity
  • EAT
  • Experimental autoimmune thyroiditis
  • In vivo animal models
  • SEA
  • Superantigens
  • T lymphocytes
  • Thyroglobulin
  • Vβ1
  • Vβ13

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Participation of Vβ13<sup>+</sup> and Vβ1<sup>+</sup> T cells in transfer thyroiditis after activation of mouse thyroglobulin-primed T cells by superantigen staphylococcal enterotoxin A'. Together they form a unique fingerprint.

  • Cite this

    Wan, Q., Kita, M., Flynn, J. C., Panos, J. C., Motte, R. W., Davies, T. F., Giraldo, A. A., David, C. S., & Kong, Y. C. M. (2001). Participation of Vβ13+ and Vβ1+ T cells in transfer thyroiditis after activation of mouse thyroglobulin-primed T cells by superantigen staphylococcal enterotoxin A. Cellular Immunology, 213(2), 149-157. https://doi.org/10.1006/cimm.2001.1875