Paracrine production of nerve growth factor during rat dorsal root ganglion development

Jagjit S. Gill, Anthony John Windebank

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nerve growth factor is a target derived growth factor. In the peripheral nervous system, it is produced by tissues innervated by the sympathetic nervous system and small sensory neurons. In the present study, we tested the hypothesis that an alternate source of nerve growth factor must be available to support dorsal root ganglion neurons before they make connection with the target. Using reverse transcriptase polymerase chain reaction (RT-PCR), we detected nerve growth factor mRNA at embryonic day 12 to 17, but not in adult dorsal root ganglia. In situ hybridization studies revealed positive staining in satellite/supportive cells juxtaposed to dorsal root ganglion neurons. Our study suggests that nerve growth factor from supporting cells may have a paracrine function during development of primary sensory neurons.

Original languageEnglish (US)
Pages (from-to)149-152
Number of pages4
JournalNeuroscience Letters
Volume251
Issue number3
DOIs
StatePublished - Jul 31 1998

Fingerprint

Spinal Ganglia
Nerve Growth Factor
Sensory Receptor Cells
Neurons
Sympathetic Nervous System
Peripheral Nervous System
Reverse Transcriptase Polymerase Chain Reaction
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Staining and Labeling
Messenger RNA

Keywords

  • Development
  • Dorsal root ganglion
  • Neurotrophin
  • Paracrine
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Paracrine production of nerve growth factor during rat dorsal root ganglion development. / Gill, Jagjit S.; Windebank, Anthony John.

In: Neuroscience Letters, Vol. 251, No. 3, 31.07.1998, p. 149-152.

Research output: Contribution to journalArticle

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