Osteogenic potential of human adipose-tissue-derived mesenchymal stromal cells cultured on 3D-printed porous structured titanium

Eric A. Lewallen, Dakota L. Jones, Amel Dudakovic, Roman Thaler, Christopher R. Paradise, Hilal D Maradit Kremers, Matthew Abdel, Sanjeev Kakar, Allan B Dietz, Robert C. Cohen, David G. Lewallen, Andre J van Wijnen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Integration of porous metal prosthetics, which restore form and function of irreversibly damaged joints, into remaining healthy bone is critical for implant success. We investigated the biological properties of adipose-tissue-derived mesenchymal stromal/stem cells (AMSCs) and addressed their potential to alter the in vitro microenvironment of implants. We employed human AMSCs as a practical source for musculoskeletal applications because these cells can be obtained in large quantities, are multipotent, and have trophic paracrine functions. AMSCs were cultured on surgical-grade porous titanium disks as a model for orthopedic implants. We monitored cell/substrate attachment, cell proliferation, multipotency, and differentiation phenotypes of AMSCs upon osteogenic induction. High-resolution scanning electron microscopy and histology revealed that AMSCs adhere to the porous metallic surface. Compared to standard tissue culture plastic, AMSCs grown in the porous titanium microenvironment showed differences in temporal expression for genes involved in cell cycle progression (CCNB2, HIST2H4), extracellular matrix production (COL1A1, COL3A1), mesenchymal lineage identity (ACTA2, CD248, CD44), osteoblastic transcription factors (DLX3, DLX5, ID3), and epigenetic regulators (EZH1, EZH2). We conclude that metal orthopedic implants can be effectively seeded with clinical-grade stem/stromal cells to create a pre-conditioned implant.

Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalGene
Volume581
Issue number2
DOIs
StatePublished - May 1 2016

Fingerprint

Titanium
Mesenchymal Stromal Cells
Adipose Tissue
Orthopedics
Metals
Stromal Cells
Epigenomics
Electron Scanning Microscopy
Plastics
Extracellular Matrix
Cell Differentiation
Histology
Cell Cycle
Transcription Factors
Stem Cells
Joints
Cell Proliferation
Phenotype
Gene Expression
Bone and Bones

Keywords

  • Biomaterial
  • Extracellular matrix
  • Gene expression
  • Mesenchymal stem cell
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Genetics

Cite this

Osteogenic potential of human adipose-tissue-derived mesenchymal stromal cells cultured on 3D-printed porous structured titanium. / Lewallen, Eric A.; Jones, Dakota L.; Dudakovic, Amel; Thaler, Roman; Paradise, Christopher R.; Maradit Kremers, Hilal D; Abdel, Matthew; Kakar, Sanjeev; Dietz, Allan B; Cohen, Robert C.; Lewallen, David G.; van Wijnen, Andre J.

In: Gene, Vol. 581, No. 2, 01.05.2016, p. 95-106.

Research output: Contribution to journalArticle

Lewallen, Eric A. ; Jones, Dakota L. ; Dudakovic, Amel ; Thaler, Roman ; Paradise, Christopher R. ; Maradit Kremers, Hilal D ; Abdel, Matthew ; Kakar, Sanjeev ; Dietz, Allan B ; Cohen, Robert C. ; Lewallen, David G. ; van Wijnen, Andre J. / Osteogenic potential of human adipose-tissue-derived mesenchymal stromal cells cultured on 3D-printed porous structured titanium. In: Gene. 2016 ; Vol. 581, No. 2. pp. 95-106.
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