Nonphotochemical hole burning spectroscopy of a mitochondrial selective rhodamine dye molecule in normal and cancerous ovarian surface epithelial cells

R. J. Walsh, T. Reinot, J. M. Hayes, K. R. Kalli, L. C. Hartmann, G. J. Small

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Results are presented of nonphotochemical hole burning experiments on the mitochondrial dye rhodamine 800 (MitoFluor Far Red 680, Molecular Probes) incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV 167 carcinoma (cancer) cells. This dye is believed to be selective for the plasma and inner membranes of the mitochondria. Importantly, the dispersive (distributed) growth kinetics of zero-phonon holes were found to be significantly different with the OV 167 line exhibiting the higher burn efficiency. This may reflect greater structural heterogeneity of that line. Equally interesting is that the permanent dipole moment change (Δμ) for the S 0 → S 1 transition of the dye is markedly different for the two cell lines, the Δμ value for the carcinoma cell line being a factor of 1.5 higher. Discussion of this finding in terms of membrane potentials is given.

Original languageEnglish (US)
Pages (from-to)115-121
Number of pages7
JournalJournal of Luminescence
Volume98
Issue number1-4
DOIs
StatePublished - Jul 2002

Fingerprint

Rhodamines
hole burning
rhodamine
cultured cells
Spectrum Analysis
Coloring Agents
dyes
cancer
Epithelial Cells
Spectroscopy
Cell Line
Molecules
Phonons
Cells
spectroscopy
membranes
Membranes
Carcinoma
molecules
Molecular Probes

Keywords

  • Carcinoma
  • Hole burning
  • Membrane potential
  • Mitochondria
  • Stark effect

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Atomic and Molecular Physics, and Optics

Cite this

Nonphotochemical hole burning spectroscopy of a mitochondrial selective rhodamine dye molecule in normal and cancerous ovarian surface epithelial cells. / Walsh, R. J.; Reinot, T.; Hayes, J. M.; Kalli, K. R.; Hartmann, L. C.; Small, G. J.

In: Journal of Luminescence, Vol. 98, No. 1-4, 07.2002, p. 115-121.

Research output: Contribution to journalArticle

Walsh, R. J. ; Reinot, T. ; Hayes, J. M. ; Kalli, K. R. ; Hartmann, L. C. ; Small, G. J. / Nonphotochemical hole burning spectroscopy of a mitochondrial selective rhodamine dye molecule in normal and cancerous ovarian surface epithelial cells. In: Journal of Luminescence. 2002 ; Vol. 98, No. 1-4. pp. 115-121.
@article{58b6c2667e9b44d6b74d9fd4f12025a7,
title = "Nonphotochemical hole burning spectroscopy of a mitochondrial selective rhodamine dye molecule in normal and cancerous ovarian surface epithelial cells",
abstract = "Results are presented of nonphotochemical hole burning experiments on the mitochondrial dye rhodamine 800 (MitoFluor Far Red 680, Molecular Probes) incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV 167 carcinoma (cancer) cells. This dye is believed to be selective for the plasma and inner membranes of the mitochondria. Importantly, the dispersive (distributed) growth kinetics of zero-phonon holes were found to be significantly different with the OV 167 line exhibiting the higher burn efficiency. This may reflect greater structural heterogeneity of that line. Equally interesting is that the permanent dipole moment change (Δμ) for the S 0 → S 1 transition of the dye is markedly different for the two cell lines, the Δμ value for the carcinoma cell line being a factor of 1.5 higher. Discussion of this finding in terms of membrane potentials is given.",
keywords = "Carcinoma, Hole burning, Membrane potential, Mitochondria, Stark effect",
author = "Walsh, {R. J.} and T. Reinot and Hayes, {J. M.} and Kalli, {K. R.} and Hartmann, {L. C.} and Small, {G. J.}",
year = "2002",
month = "7",
doi = "10.1016/S0022-2313(02)00259-4",
language = "English (US)",
volume = "98",
pages = "115--121",
journal = "Journal of Luminescence",
issn = "0022-2313",
publisher = "Elsevier",
number = "1-4",

}

TY - JOUR

T1 - Nonphotochemical hole burning spectroscopy of a mitochondrial selective rhodamine dye molecule in normal and cancerous ovarian surface epithelial cells

AU - Walsh, R. J.

AU - Reinot, T.

AU - Hayes, J. M.

AU - Kalli, K. R.

AU - Hartmann, L. C.

AU - Small, G. J.

PY - 2002/7

Y1 - 2002/7

N2 - Results are presented of nonphotochemical hole burning experiments on the mitochondrial dye rhodamine 800 (MitoFluor Far Red 680, Molecular Probes) incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV 167 carcinoma (cancer) cells. This dye is believed to be selective for the plasma and inner membranes of the mitochondria. Importantly, the dispersive (distributed) growth kinetics of zero-phonon holes were found to be significantly different with the OV 167 line exhibiting the higher burn efficiency. This may reflect greater structural heterogeneity of that line. Equally interesting is that the permanent dipole moment change (Δμ) for the S 0 → S 1 transition of the dye is markedly different for the two cell lines, the Δμ value for the carcinoma cell line being a factor of 1.5 higher. Discussion of this finding in terms of membrane potentials is given.

AB - Results are presented of nonphotochemical hole burning experiments on the mitochondrial dye rhodamine 800 (MitoFluor Far Red 680, Molecular Probes) incubated with two human ovarian surface epithelial cell lines: OSE(tsT)-14 normal cells and OV 167 carcinoma (cancer) cells. This dye is believed to be selective for the plasma and inner membranes of the mitochondria. Importantly, the dispersive (distributed) growth kinetics of zero-phonon holes were found to be significantly different with the OV 167 line exhibiting the higher burn efficiency. This may reflect greater structural heterogeneity of that line. Equally interesting is that the permanent dipole moment change (Δμ) for the S 0 → S 1 transition of the dye is markedly different for the two cell lines, the Δμ value for the carcinoma cell line being a factor of 1.5 higher. Discussion of this finding in terms of membrane potentials is given.

KW - Carcinoma

KW - Hole burning

KW - Membrane potential

KW - Mitochondria

KW - Stark effect

UR - http://www.scopus.com/inward/record.url?scp=0036649125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036649125&partnerID=8YFLogxK

U2 - 10.1016/S0022-2313(02)00259-4

DO - 10.1016/S0022-2313(02)00259-4

M3 - Article

AN - SCOPUS:0036649125

VL - 98

SP - 115

EP - 121

JO - Journal of Luminescence

JF - Journal of Luminescence

SN - 0022-2313

IS - 1-4

ER -