Noninvasive Contrast-Free 3D Evaluation of Tumor Angiogenesis with Ultrasensitive Ultrasound Microvessel Imaging

Chengwu Huang, Matthew R. Lowerison, Fabrice Lucien, Ping Gong, Diping Wang, Pengfei Song, Shigao D Chen

Research output: Contribution to journalArticle

Abstract

Ultrasound microvessel imaging (UMI), when applied with ultrafast planewave acquisitions, has demonstrated superior blood signal sensitivity in comparison to conventional Doppler imaging. Here we propose a high spatial resolution and ultra-sensitive UMI that is based on conventional line-by-line high-frequency ultrasound imagers and singular value decomposition (SVD) clutter filtering for the visualization and quantification of tumor microvasculature and perfusion. The technique was applied to a chicken embryo tumor model of renal cell carcinoma that was treated with two FDA-approved anti-angiogenic agents at clinically relevant dosages. We demonstrate the feasibility of 3D evaluation with UMI to achieve highly sensitive detection of microvasculature using conventional line-by-line ultrasound imaging on a preclinical and commercially available high-frequency ultrasound device without software or hardware modifications. Quantitative parameters (vascularization index and fractional moving blood volume) derived from UMI images provide significantly improved evaluation of anti-angiogenic therapy response as compared with conventional power Doppler imaging, using histological analysis and immunohistochemistry as the reference standard. This proof-of-concept study demonstrates that high-frequency UMI is a low-cost, contrast-agent-free, easily applicable, accessible, and quantitative imaging tool for tumor characterization, which may be very useful for preclinical evaluation and longitudinal monitoring of anti-cancer treatment.

Original languageEnglish (US)
Article number4907
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Microvessels
Ultrasonography
Neoplasms
Blood Volume
Renal Cell Carcinoma
Contrast Media
Chickens
Software
Embryonic Structures
Perfusion
Immunohistochemistry
Costs and Cost Analysis
Equipment and Supplies
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Noninvasive Contrast-Free 3D Evaluation of Tumor Angiogenesis with Ultrasensitive Ultrasound Microvessel Imaging. / Huang, Chengwu; Lowerison, Matthew R.; Lucien, Fabrice; Gong, Ping; Wang, Diping; Song, Pengfei; Chen, Shigao D.

In: Scientific reports, Vol. 9, No. 1, 4907, 01.12.2019.

Research output: Contribution to journalArticle

Huang, Chengwu ; Lowerison, Matthew R. ; Lucien, Fabrice ; Gong, Ping ; Wang, Diping ; Song, Pengfei ; Chen, Shigao D. / Noninvasive Contrast-Free 3D Evaluation of Tumor Angiogenesis with Ultrasensitive Ultrasound Microvessel Imaging. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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