Nitric oxide induces apoptosis in mouse C2C12 myoblast cells

Myoung Hwa Lee, Mi-Hyeon D Jang, Eun Kyung Kim, Sang Wan Han, Seong Yeon Cho, Chang Ju Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To investigate whether nitric oxide (NO) induces apoptosis in myoblast cells, the effect of the sodium nitroprusside (SNP), NO donor, on the apoptosis of mouse C2C12 myoblast cells was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2- phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme assay. Mouse C2C12 myoblast cells treated with SNP exhibited several apoptotic features. SNP increased p53 expression and bax expression. SNP also enhanced caspase-3 enzyme activity. The data show that NO may induce apoptotic cell death in myoblast cells through the activation of p53-, bax-, and caspase-dependent intracellular death-related pathways.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalJournal of Pharmacological Sciences
Volume97
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

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Myoblasts
Nitroprusside
Nitric Oxide
Apoptosis
Caspase 3
Nitric Oxide Donors
DNA Nucleotidylexotransferase
Enzyme Assays
DNA Fragmentation
Caspases
Reverse Transcription
Flow Cytometry
Cell Death
Western Blotting
Staining and Labeling
Polymerase Chain Reaction
Enzymes

Keywords

  • Apoptosis
  • C2C12 myoblast cell
  • Nitric oxide

ASJC Scopus subject areas

  • Pharmacology

Cite this

Nitric oxide induces apoptosis in mouse C2C12 myoblast cells. / Lee, Myoung Hwa; Jang, Mi-Hyeon D; Kim, Eun Kyung; Han, Sang Wan; Cho, Seong Yeon; Kim, Chang Ju.

In: Journal of Pharmacological Sciences, Vol. 97, No. 3, 03.2005, p. 369-376.

Research output: Contribution to journalArticle

Lee, Myoung Hwa ; Jang, Mi-Hyeon D ; Kim, Eun Kyung ; Han, Sang Wan ; Cho, Seong Yeon ; Kim, Chang Ju. / Nitric oxide induces apoptosis in mouse C2C12 myoblast cells. In: Journal of Pharmacological Sciences. 2005 ; Vol. 97, No. 3. pp. 369-376.
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