Nitric oxide induces apoptosis in mouse C2C12 myoblast cells

Myoung Hwa Lee, Mi Hyeon Jang, Eun Kyung Kim, Sang Wan Han, Seong Yeon Cho, Chang Ju Kim

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

To investigate whether nitric oxide (NO) induces apoptosis in myoblast cells, the effect of the sodium nitroprusside (SNP), NO donor, on the apoptosis of mouse C2C12 myoblast cells was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2- phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme assay. Mouse C2C12 myoblast cells treated with SNP exhibited several apoptotic features. SNP increased p53 expression and bax expression. SNP also enhanced caspase-3 enzyme activity. The data show that NO may induce apoptotic cell death in myoblast cells through the activation of p53-, bax-, and caspase-dependent intracellular death-related pathways.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalJournal of Pharmacological Sciences
Volume97
Issue number3
DOIs
StatePublished - Mar 2005

Keywords

  • Apoptosis
  • C2C12 myoblast cell
  • Nitric oxide

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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