New mouse model for studying laryngeal transplantation

Taha Z. Shipchandler, David G Lott, Robert R. Lorenz, Aaron D. Friedman, Olivia Dan, Marshall Strome

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives: Laryngeal transplantation research has been studied in various animal models. For in-depth, immunology-based transplantation research, however, a thoroughly studied animal model must exist. The purpose of this study was to develop a reliable surgical technique in mice to serve as a model for further study of laryngeal transplantation. Methods: Heterotopic laryngeal transplantation was attempted in 15 immunocompetent mice by use of modifications of previously described techniques established in rats. Results: Various microvascular techniques were used that led to 8 successful transplants (of 15) with patent vascularity at the time of sacrifice. The first 7 attempts at transplantation were unsuccessful because of technical difficulties related to vessel size, soft tissue traumatic injury, and venous congestion. Subsequently, 8 transplantation procedures were successfully performed after modifications of the surgical technique. Conclusions: This pilot study describes the reproducible surgical techniques performed in using mice for studying laryngeal transplantation. Mice are cost-effective and immunologically well studied, and are thus ideal for further laryngeal transplantation research.

Original languageEnglish (US)
Pages (from-to)465-468
Number of pages4
JournalAnnals of Otology, Rhinology and Laryngology
Volume118
Issue number6
StatePublished - Jun 2009
Externally publishedYes

Fingerprint

Transplantation
Transplantation Immunology
Animal Models
Research
Heterotopic Transplantation
Soft Tissue Injuries
Hyperemia
Transplants
Costs and Cost Analysis

Keywords

  • Larynx
  • Microvascular surgery
  • Mouse
  • Transplantation

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Shipchandler, T. Z., Lott, D. G., Lorenz, R. R., Friedman, A. D., Dan, O., & Strome, M. (2009). New mouse model for studying laryngeal transplantation. Annals of Otology, Rhinology and Laryngology, 118(6), 465-468.

New mouse model for studying laryngeal transplantation. / Shipchandler, Taha Z.; Lott, David G; Lorenz, Robert R.; Friedman, Aaron D.; Dan, Olivia; Strome, Marshall.

In: Annals of Otology, Rhinology and Laryngology, Vol. 118, No. 6, 06.2009, p. 465-468.

Research output: Contribution to journalArticle

Shipchandler, TZ, Lott, DG, Lorenz, RR, Friedman, AD, Dan, O & Strome, M 2009, 'New mouse model for studying laryngeal transplantation', Annals of Otology, Rhinology and Laryngology, vol. 118, no. 6, pp. 465-468.
Shipchandler TZ, Lott DG, Lorenz RR, Friedman AD, Dan O, Strome M. New mouse model for studying laryngeal transplantation. Annals of Otology, Rhinology and Laryngology. 2009 Jun;118(6):465-468.
Shipchandler, Taha Z. ; Lott, David G ; Lorenz, Robert R. ; Friedman, Aaron D. ; Dan, Olivia ; Strome, Marshall. / New mouse model for studying laryngeal transplantation. In: Annals of Otology, Rhinology and Laryngology. 2009 ; Vol. 118, No. 6. pp. 465-468.
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