Abstract
Objectives: Laryngeal transplantation research has been studied in various animal models. For in-depth, immunology-based transplantation research, however, a thoroughly studied animal model must exist. The purpose of this study was to develop a reliable surgical technique in mice to serve as a model for further study of laryngeal transplantation. Methods: Heterotopic laryngeal transplantation was attempted in 15 immunocompetent mice by use of modifications of previously described techniques established in rats. Results: Various microvascular techniques were used that led to 8 successful transplants (of 15) with patent vascularity at the time of sacrifice. The first 7 attempts at transplantation were unsuccessful because of technical difficulties related to vessel size, soft tissue traumatic injury, and venous congestion. Subsequently, 8 transplantation procedures were successfully performed after modifications of the surgical technique. Conclusions: This pilot study describes the reproducible surgical techniques performed in using mice for studying laryngeal transplantation. Mice are cost-effective and immunologically well studied, and are thus ideal for further laryngeal transplantation research.
Original language | English (US) |
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Pages (from-to) | 465-468 |
Number of pages | 4 |
Journal | Annals of Otology, Rhinology and Laryngology |
Volume | 118 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2009 |
Keywords
- Larynx
- Microvascular surgery
- Mouse
- Transplantation
ASJC Scopus subject areas
- Otorhinolaryngology