New mouse model for studying laryngeal transplantation

Taha Z. Shipchandler, David G. Lott, Robert R. Lorenz, Aaron D. Friedman, Olivia Dan, Marshall Strome

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Objectives: Laryngeal transplantation research has been studied in various animal models. For in-depth, immunology-based transplantation research, however, a thoroughly studied animal model must exist. The purpose of this study was to develop a reliable surgical technique in mice to serve as a model for further study of laryngeal transplantation. Methods: Heterotopic laryngeal transplantation was attempted in 15 immunocompetent mice by use of modifications of previously described techniques established in rats. Results: Various microvascular techniques were used that led to 8 successful transplants (of 15) with patent vascularity at the time of sacrifice. The first 7 attempts at transplantation were unsuccessful because of technical difficulties related to vessel size, soft tissue traumatic injury, and venous congestion. Subsequently, 8 transplantation procedures were successfully performed after modifications of the surgical technique. Conclusions: This pilot study describes the reproducible surgical techniques performed in using mice for studying laryngeal transplantation. Mice are cost-effective and immunologically well studied, and are thus ideal for further laryngeal transplantation research.

Original languageEnglish (US)
Pages (from-to)465-468
Number of pages4
JournalAnnals of Otology, Rhinology and Laryngology
Volume118
Issue number6
DOIs
StatePublished - Jun 2009

Keywords

  • Larynx
  • Microvascular surgery
  • Mouse
  • Transplantation

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Shipchandler, T. Z., Lott, D. G., Lorenz, R. R., Friedman, A. D., Dan, O., & Strome, M. (2009). New mouse model for studying laryngeal transplantation. Annals of Otology, Rhinology and Laryngology, 118(6), 465-468. https://doi.org/10.1177/000348940911800610