@article{c3ce883906de42ba962edd8ae7676090,
title = "NEAT-seq: simultaneous profiling of intra-nuclear proteins, chromatin accessibility and gene expression in single cells",
abstract = "In this work, we describe NEAT-seq (sequencing of nuclear protein epitope abundance, chromatin accessibility and the transcriptome in single cells), enabling interrogation of regulatory mechanisms spanning the central dogma. We apply this technique to profile CD4 memory T cells using a panel of master transcription factors (TFs) that drive T cell subsets and identify examples of TFs with regulatory activity gated by transcription, translation and regulation of chromatin binding. We also link a noncoding genome-wide association study single-nucleotide polymorphism (SNP) within a GATA motif to a putative target gene, using NEAT-seq data to internally validate SNP impact on GATA3 regulation.",
author = "Chen, {Amy F.} and Benjamin Parks and Kathiria, {Arwa S.} and Benjamin Ober-Reynolds and Goronzy, {Jorg J.} and Greenleaf, {William J.}",
note = "Funding Information: We thank R. Baskar, S. Pierce, S. Klemm, Y. Kim and members of the Greenleaf laboratory for helpful discussions and suggestions. We also thank the Stanford Fluorescence Activated Cell Sorting facility and Stanford Functional Genomics Facility for technical support. Figure schematics were created with BioRender.com. This work was supported by funding from the National Institutes of Health (NIH) (grant nos. P50HG007735, R01HG008140, R01HG009909, UM1HG011972, U01MH116529, U54HG010426, U01DK127419, U01HG011762, U19AI057266 and UM1HG009442), the Rita Allen Foundation, the Baxter Foundation Faculty Scholar Grant and the Human Frontiers Science Program (grant no. RGY006S) to W.J.G. W.J.G. is a Chan Zuckerberg Biohub investigator (grant nos. 2017-174468 and 2018-182817). Fellowship support was provided by the Stanford School of Medicine Dean{\textquoteright}s Fellowship and NIH (grant no. F32GM135996) to A.F.C. and a training grant from the National Institute of Standards and Technology to B.P. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2022",
month = may,
doi = "10.1038/s41592-022-01461-y",
language = "English (US)",
volume = "19",
pages = "547--553",
journal = "PLoS Medicine",
issn = "1549-1277",
publisher = "Nature Publishing Group",
number = "5",
}