Mutations in the pancreatic secretory enzymes CPA1 and CPB1 are associated with pancreatic cancer

Koji Tamura, Jun Yu, Tatsuo Hata, Masaya Suenaga, Koji Shindo, Toshiya Abe, Anne MacGregor-Das, Michael Borges, Christopher L. Wolfgang, Matthew J. Weiss, Jin He, Marcia Irene Canto, Gloria M. Petersen, Steven Gallinger, Sapna Syngal, Randall E. Brand, Anil Rustgi, Sara H. Olson, Elena Stoffel, Michele L. CoteGeorge Zogopoulos, James B. Potash, Fernando S. Goes, Richard W. McCombie, Peter P. Zandi, Mehdi Pirooznia, Melissa Kramer, Jennifer Parla, James R. Eshleman, Nicholas J. Roberts, Ralph H. Hruban, Alison Patricia Klein, Michael Goggins

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

To evaluate whether germline variants in genes encoding pancreatic secretory enzymes contribute to pancreatic cancer susceptibility, we sequenced the coding regions of CPB1 and other genes encoding pancreatic secretory enzymes and known pancreatitis susceptibility genes (PRSS1, CPA1, CTRC, and SPINK1) in a hospital series of pancreatic cancer cases and controls. Variants in CPB1, CPA1 (encoding carboxypeptidase B1 and A1), and CTRC were evaluated in a second set of cases with familial pancreatic cancer and controls. More deleterious CPB1 variants, defined as having impaired protein secretion and induction of endoplasmic reticulum (ER) stress in transfected HEK 293T cells, were found in the hospital series of pancreatic cancer cases (5/986, 0.5%) than in controls (0/1,045, P = 0.027). Among familial pancreatic cancer cases, ER stress-inducing CPB1 variants were found in 4 of 593 (0.67%) vs. 0 of 967 additional controls (P = 0.020), with a combined prevalence in pancreatic cancer cases of 9/1,579 vs. 0/2,012 controls (P < 0.01). More ER stress-inducing CPA1 variants were also found in the combined set of hospital and familial cases with pancreatic cancer than in controls [7/1,546 vs. 1/2,012; P = 0.025; odds ratio, 9.36 (95% CI, 1.15–76.02)]. Overall, 16 (1%) of 1,579 pancreatic cancer cases had an ER stress-inducing CPA1 or CPB1 variant, compared with 1 of 2,068 controls (P < 0.00001). No other candidate genes had statistically significant differences in variant prevalence between cases and controls. Our study indicates ER stress-inducing variants in CPB1 and CPA1 are associated with pancreatic cancer susceptibility and implicate ER stress in pancreatic acinar cells in pancreatic cancer development.

Original languageEnglish (US)
Pages (from-to)4767-4772
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number18
DOIs
StatePublished - May 1 2018

Keywords

  • CPA1
  • CPB1
  • ER stress
  • Pancreatic cancer
  • Pancreatitis

ASJC Scopus subject areas

  • General

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