Muscle creatine kinase-deficient mice: II. Cardiac and skeletal muscles exhibit tissue-specific adaptation of the mitochondrial function

Vladimir I. Veksler, Andrey V. Kuznetsov, Keltoum Anflous, Philippe Mateo, Jan Van Deursen, Bé Wieringa, Renée Ventura-Clapier

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Functional properties of in situ mitochondria and of mitochondrial creatine kinase were studied in saponin-skinned fibers taken from normal and M-creatine kinase-deficient mice. In control animals, apparent Km values of mitochondrial respiration for ADP in cardiac (ventricular) and slow-twitch (soleus) muscles (137 ± 16 μM and 209 ± 10 μM, respectively) were manyfold higher than that in fast-twitch (gastrocnemius) muscle (7.5 ± 0.5 μM). Creatine substantially decreased the Km values only in cardiac and slow-twitch muscles (73 ± 11 μM and 131 ± 21 μM, respectively). As compared to control, in situ mitochondria in transgenic ventricular and slow-twitch muscles showed two times lower Km values for ADP, and the presence of creatine only slightly decreased the Km values. In mutant fast-twitch muscle, a decrease rather than increase in mitochondrial sensitivity to ADP occurred, but creatine still had no effect. Furthermore, in these muscles, relatively low oxidative capacity was considerably elevated. It is suggested that in the mutant mice, impairment of energy transport function in ventricular and slow-twitch muscles is compensated by a facilitation of adenine nucleotide transportation between mitochondria and cellular ATPases; in fast-twitch muscle, mainly energy buffering function is depressed, and that is overcome by an increase in energy-producing potential.

Original languageEnglish (US)
Pages (from-to)19921-19929
Number of pages9
JournalJournal of Biological Chemistry
Volume270
Issue number34
StatePublished - Aug 25 1995
Externally publishedYes

Fingerprint

MM Form Creatine Kinase
Muscle
Myocardium
Skeletal Muscle
Tissue
Muscles
Creatine
Adenosine Diphosphate
Mitochondria
Mitochondrial Form Creatine Kinase
Ventricular Function
Adenine Nucleotides
Saponins
Adenosine Triphosphatases
Respiration
Potential energy
Animals
Fibers

ASJC Scopus subject areas

  • Biochemistry

Cite this

Veksler, V. I., Kuznetsov, A. V., Anflous, K., Mateo, P., Van Deursen, J., Wieringa, B., & Ventura-Clapier, R. (1995). Muscle creatine kinase-deficient mice: II. Cardiac and skeletal muscles exhibit tissue-specific adaptation of the mitochondrial function. Journal of Biological Chemistry, 270(34), 19921-19929.

Muscle creatine kinase-deficient mice : II. Cardiac and skeletal muscles exhibit tissue-specific adaptation of the mitochondrial function. / Veksler, Vladimir I.; Kuznetsov, Andrey V.; Anflous, Keltoum; Mateo, Philippe; Van Deursen, Jan; Wieringa, Bé; Ventura-Clapier, Renée.

In: Journal of Biological Chemistry, Vol. 270, No. 34, 25.08.1995, p. 19921-19929.

Research output: Contribution to journalArticle

Veksler, VI, Kuznetsov, AV, Anflous, K, Mateo, P, Van Deursen, J, Wieringa, B & Ventura-Clapier, R 1995, 'Muscle creatine kinase-deficient mice: II. Cardiac and skeletal muscles exhibit tissue-specific adaptation of the mitochondrial function', Journal of Biological Chemistry, vol. 270, no. 34, pp. 19921-19929.
Veksler, Vladimir I. ; Kuznetsov, Andrey V. ; Anflous, Keltoum ; Mateo, Philippe ; Van Deursen, Jan ; Wieringa, Bé ; Ventura-Clapier, Renée. / Muscle creatine kinase-deficient mice : II. Cardiac and skeletal muscles exhibit tissue-specific adaptation of the mitochondrial function. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 34. pp. 19921-19929.
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