mRNA expression in rabbit experimental aneurysms: A study using gene chip microarrays

W. I. Mangrum, F. Farassati, Ramanathan D Kadirvel, C. P. Kolbert, S. Raghavakaimal, D. Dai, Y. H. Ding, D. Grill, V. G. Khurana, David F Kallmes

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE: The molecular characteristics of intracranial aneurysms are still poorly documented. A rabbit elastase aneurysm model has been helpful in the evaluation of devices and strategies involved in endovascular treatment of aneurysms. The goal of this project was to document the molecular changes, assessed by gene chip microarrays, associated with the creation of aneurysms in this model compared with the contralateral carotid artery. MATERIALS AND METHODS: A microarray of rabbit genes of interest was constructed using rabbit nucleotide sequences from GenBank. Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 4 rabbits. Twelve weeks after aneurysm creation, RNA was isolated from the aneurysm as well as the contralateral common carotid artery and used for microarray experiments. Reverse transcription-polymerase chain reaction (RT-PCR) was performed on 1 animal as a confirmatory test. RESULTS: Ninety-six (46%) of 209 genes in the microarray were differentially expressed in the rabbit aneurysm compared with the contralateral common carotid artery. In general, differential gene expression followed specific molecular pathways. Similarities were found between rabbit aneurysms and human intracranial aneurysms, including increased metalloproteinase activity and decreased production of the extracellular matrix. RT-PCR results confirmed the differential expression found by the gene chip microarray. CONCLUSIONS: The molecular characteristics of the rabbit elastase-induced saccular aneurysm are described. The rabbit aneurysm model shares some molecular features with human intracranial aneurysms. Future studies can use the rabbit model and the new rabbit gene chip microarray to study the molecular aspects of saccular aneurysms.

Original languageEnglish (US)
Pages (from-to)864-869
Number of pages6
JournalAmerican Journal of Neuroradiology
Volume28
Issue number5
StatePublished - May 2007

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Oligonucleotide Array Sequence Analysis
Aneurysm
Rabbits
Messenger RNA
Pancreatic Elastase
Common Carotid Artery
Intracranial Aneurysm
Reverse Transcription
Polymerase Chain Reaction
Nucleic Acid Databases
Metalloproteases
Carotid Arteries
Genes
Extracellular Matrix
RNA
Gene Expression
Equipment and Supplies

ASJC Scopus subject areas

  • Clinical Neurology
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

mRNA expression in rabbit experimental aneurysms : A study using gene chip microarrays. / Mangrum, W. I.; Farassati, F.; Kadirvel, Ramanathan D; Kolbert, C. P.; Raghavakaimal, S.; Dai, D.; Ding, Y. H.; Grill, D.; Khurana, V. G.; Kallmes, David F.

In: American Journal of Neuroradiology, Vol. 28, No. 5, 05.2007, p. 864-869.

Research output: Contribution to journalArticle

Mangrum, WI, Farassati, F, Kadirvel, RD, Kolbert, CP, Raghavakaimal, S, Dai, D, Ding, YH, Grill, D, Khurana, VG & Kallmes, DF 2007, 'mRNA expression in rabbit experimental aneurysms: A study using gene chip microarrays', American Journal of Neuroradiology, vol. 28, no. 5, pp. 864-869.
Mangrum WI, Farassati F, Kadirvel RD, Kolbert CP, Raghavakaimal S, Dai D et al. mRNA expression in rabbit experimental aneurysms: A study using gene chip microarrays. American Journal of Neuroradiology. 2007 May;28(5):864-869.
Mangrum, W. I. ; Farassati, F. ; Kadirvel, Ramanathan D ; Kolbert, C. P. ; Raghavakaimal, S. ; Dai, D. ; Ding, Y. H. ; Grill, D. ; Khurana, V. G. ; Kallmes, David F. / mRNA expression in rabbit experimental aneurysms : A study using gene chip microarrays. In: American Journal of Neuroradiology. 2007 ; Vol. 28, No. 5. pp. 864-869.
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