TY - JOUR
T1 - MR histological correlation
T2 - A method for cutting specimens along the imaging plane in animal or ex vivo experiments
AU - Rouvière, Olivier
AU - Reynolds, Carol
AU - Hulshizer, Thomas
AU - Rossman, Phillip
AU - Le, Yuan
AU - Felmlee, Joel P.
AU - Ehman, Richard L.
PY - 2006/1
Y1 - 2006/1
N2 - Purpose: To assess a method aimed at cutting histological specimens along the magnetic resonance (MR) imaging plane. Material and Methods: The method is performed in two steps: the imaging plane (defined by three acrylic paint markers) is made horizontal under MR guidance by using a mobile platform that can be rotated in three directions (PlaneFinder device [PFD]): then, the specimen is embedded in wax and cut horizontally. Three-dimensional images parallel to the markers' plane were obtained on 31 pork muscles containing a central hole with a pyramidal shape, with a technique of reference (RT images) and with PFD (PF images), before and after fixation. The last 17 fixed specimens were cut in the markers' plane (tissue section [TS] images). The central hole area (CHA) in the markers' plane was used to compare RT, PF, and TS images. Using a workstation, PF images were rotated and translated to estimate the shift along each direction that could explain the entire CHA difference between RT, PF, and TS images (maximum error, worst-case scenario). Results: Excellent correlation was found between RT and PF images (r = 0.989, slope = 1.0175), PF and TS images (r = 0.991, slope = 1.0058), and RT images on fresh specimens and TS images (r = 0.979, slope = 1.0732). For each step, the maximum angle error was ≤ 3° in 88-95% of the specimens. Conclusion: Our methodology can be used to cut specimens along the imaging plane with high accuracy.
AB - Purpose: To assess a method aimed at cutting histological specimens along the magnetic resonance (MR) imaging plane. Material and Methods: The method is performed in two steps: the imaging plane (defined by three acrylic paint markers) is made horizontal under MR guidance by using a mobile platform that can be rotated in three directions (PlaneFinder device [PFD]): then, the specimen is embedded in wax and cut horizontally. Three-dimensional images parallel to the markers' plane were obtained on 31 pork muscles containing a central hole with a pyramidal shape, with a technique of reference (RT images) and with PFD (PF images), before and after fixation. The last 17 fixed specimens were cut in the markers' plane (tissue section [TS] images). The central hole area (CHA) in the markers' plane was used to compare RT, PF, and TS images. Using a workstation, PF images were rotated and translated to estimate the shift along each direction that could explain the entire CHA difference between RT, PF, and TS images (maximum error, worst-case scenario). Results: Excellent correlation was found between RT and PF images (r = 0.989, slope = 1.0175), PF and TS images (r = 0.991, slope = 1.0058), and RT images on fresh specimens and TS images (r = 0.979, slope = 1.0732). For each step, the maximum angle error was ≤ 3° in 88-95% of the specimens. Conclusion: Our methodology can be used to cut specimens along the imaging plane with high accuracy.
KW - Experimental medicine
KW - Fiducial markers
KW - Histology
KW - Image registration
KW - Magnetic resonance (MR)
UR - http://www.scopus.com/inward/record.url?scp=30744449019&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30744449019&partnerID=8YFLogxK
U2 - 10.1002/jmri.20468
DO - 10.1002/jmri.20468
M3 - Article
C2 - 16331591
AN - SCOPUS:30744449019
SN - 1053-1807
VL - 23
SP - 60
EP - 69
JO - Journal of Magnetic Resonance Imaging
JF - Journal of Magnetic Resonance Imaging
IS - 1
ER -