Molecular pathology of total knee arthroplasty instability defined by RNA-seq

Eric A. Lewallen; Christopher G. Salib; William H. Trousdale; Charlotte E. Berry; Gabrielle M. Hanssen; Joseph X. Robin; Meagan E. Tibbo; Anthony Viste; Nicolas Reina; Mark E. Morrey; Joaquin Sanchez-Sotelo; Arlen D. Hanssen; Daniel J. Berry; Andre J. van Wijnen; Matthew P. Abdel

doi:10.1016/j.ygeno.2017.11.001

Molecular pathology of total knee arthroplasty instability defined by RNA-seq

Eric A. Lewallen, Christopher G. Salib, William H. Trousdale, Charlotte E. Berry, Gabrielle M. Hanssen, Joseph X. Robin, Meagan E. Tibbo, Anthony Viste, Nicolas Reina, Mark E. Morrey, Joaquin Sanchez-Sotelo, Arlen D. Hanssen, Daniel J. Berry, Andre J. van Wijnen, Matthew P. Abdel

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.

Original language	English (US)
Pages (from-to)	247-256
Number of pages	10
Journal	Genomics
Volume	110
Issue number	5
DOIs	https://doi.org/10.1016/j.ygeno.2017.11.001
State	Published - Sep 2018

Keywords

Cell biology
Flexion instability
Molecular genetics
Revision total knee arthroplasty
Total knee arthroplasty

ASJC Scopus subject areas

Genetics

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10.1016/j.ygeno.2017.11.001

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Lewallen, E. A., Salib, C. G., Trousdale, W. H., Berry, C. E., Hanssen, G. M., Robin, J. X., Tibbo, M. E., Viste, A., Reina, N., Morrey, M. E., Sanchez-Sotelo, J., Hanssen, A. D., Berry, D. J., van Wijnen, A. J., & Abdel, M. P. (2018). Molecular pathology of total knee arthroplasty instability defined by RNA-seq. Genomics, 110(5), 247-256. https://doi.org/10.1016/j.ygeno.2017.11.001

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abstract = "Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.",

keywords = "Cell biology, Flexion instability, Molecular genetics, Revision total knee arthroplasty, Total knee arthroplasty",

author = "Lewallen, {Eric A.} and Salib, {Christopher G.} and Trousdale, {William H.} and Berry, {Charlotte E.} and Hanssen, {Gabrielle M.} and Robin, {Joseph X.} and Tibbo, {Meagan E.} and Anthony Viste and Nicolas Reina and Morrey, {Mark E.} and Joaquin Sanchez-Sotelo and Hanssen, {Arlen D.} and Berry, {Daniel J.} and {van Wijnen}, {Andre J.} and Abdel, {Matthew P.}",

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AU - Robin, Joseph X.

AU - Tibbo, Meagan E.

AU - Viste, Anthony

AU - Reina, Nicolas

AU - Morrey, Mark E.

AU - Sanchez-Sotelo, Joaquin

AU - Hanssen, Arlen D.

AU - Berry, Daniel J.

AU - van Wijnen, Andre J.

AU - Abdel, Matthew P.

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AB - Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.

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KW - Total knee arthroplasty

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Molecular pathology of total knee arthroplasty instability defined by RNA-seq

Abstract

Keywords

ASJC Scopus subject areas

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