Molecular pathology of total knee arthroplasty instability defined by RNA-seq

Eric A. Lewallen, Christopher G. Salib, William H. Trousdale, Charlotte E. Berry, Gabrielle M. Hanssen, Joseph X. Robin, Meagan E. Tibbo, Anthony Viste, Nicolas Reina, Mark E. Morrey, Joaquin Sanchez-Sotelo, Arlen D. Hanssen, Daniel J. Berry, Andre J van Wijnen, Matthew Abdel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.

Original languageEnglish (US)
JournalGenomics
DOIs
StateAccepted/In press - Jan 1 2017

Fingerprint

Knee Replacement Arthroplasties
Molecular Pathology
RNA Stability
Joint Capsule
Gene Expression
Arthralgia
Knee Joint
Extracellular Matrix
Cluster Analysis
Knee
Collagen
RNA
Genes

Keywords

  • Cell biology
  • Flexion instability
  • Molecular genetics
  • Revision total knee arthroplasty
  • Total knee arthroplasty

ASJC Scopus subject areas

  • Genetics

Cite this

Lewallen, E. A., Salib, C. G., Trousdale, W. H., Berry, C. E., Hanssen, G. M., Robin, J. X., ... Abdel, M. (Accepted/In press). Molecular pathology of total knee arthroplasty instability defined by RNA-seq. Genomics. https://doi.org/10.1016/j.ygeno.2017.11.001

Molecular pathology of total knee arthroplasty instability defined by RNA-seq. / Lewallen, Eric A.; Salib, Christopher G.; Trousdale, William H.; Berry, Charlotte E.; Hanssen, Gabrielle M.; Robin, Joseph X.; Tibbo, Meagan E.; Viste, Anthony; Reina, Nicolas; Morrey, Mark E.; Sanchez-Sotelo, Joaquin; Hanssen, Arlen D.; Berry, Daniel J.; van Wijnen, Andre J; Abdel, Matthew.

In: Genomics, 01.01.2017.

Research output: Contribution to journalArticle

Lewallen, EA, Salib, CG, Trousdale, WH, Berry, CE, Hanssen, GM, Robin, JX, Tibbo, ME, Viste, A, Reina, N, Morrey, ME, Sanchez-Sotelo, J, Hanssen, AD, Berry, DJ, van Wijnen, AJ & Abdel, M 2017, 'Molecular pathology of total knee arthroplasty instability defined by RNA-seq', Genomics. https://doi.org/10.1016/j.ygeno.2017.11.001
Lewallen EA, Salib CG, Trousdale WH, Berry CE, Hanssen GM, Robin JX et al. Molecular pathology of total knee arthroplasty instability defined by RNA-seq. Genomics. 2017 Jan 1. https://doi.org/10.1016/j.ygeno.2017.11.001
Lewallen, Eric A. ; Salib, Christopher G. ; Trousdale, William H. ; Berry, Charlotte E. ; Hanssen, Gabrielle M. ; Robin, Joseph X. ; Tibbo, Meagan E. ; Viste, Anthony ; Reina, Nicolas ; Morrey, Mark E. ; Sanchez-Sotelo, Joaquin ; Hanssen, Arlen D. ; Berry, Daniel J. ; van Wijnen, Andre J ; Abdel, Matthew. / Molecular pathology of total knee arthroplasty instability defined by RNA-seq. In: Genomics. 2017.
@article{c634bd42b4804a0084a87b34d4b038b6,
title = "Molecular pathology of total knee arthroplasty instability defined by RNA-seq",
abstract = "Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.",
keywords = "Cell biology, Flexion instability, Molecular genetics, Revision total knee arthroplasty, Total knee arthroplasty",
author = "Lewallen, {Eric A.} and Salib, {Christopher G.} and Trousdale, {William H.} and Berry, {Charlotte E.} and Hanssen, {Gabrielle M.} and Robin, {Joseph X.} and Tibbo, {Meagan E.} and Anthony Viste and Nicolas Reina and Morrey, {Mark E.} and Joaquin Sanchez-Sotelo and Hanssen, {Arlen D.} and Berry, {Daniel J.} and {van Wijnen}, {Andre J} and Matthew Abdel",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.ygeno.2017.11.001",
language = "English (US)",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Molecular pathology of total knee arthroplasty instability defined by RNA-seq

AU - Lewallen, Eric A.

AU - Salib, Christopher G.

AU - Trousdale, William H.

AU - Berry, Charlotte E.

AU - Hanssen, Gabrielle M.

AU - Robin, Joseph X.

AU - Tibbo, Meagan E.

AU - Viste, Anthony

AU - Reina, Nicolas

AU - Morrey, Mark E.

AU - Sanchez-Sotelo, Joaquin

AU - Hanssen, Arlen D.

AU - Berry, Daniel J.

AU - van Wijnen, Andre J

AU - Abdel, Matthew

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.

AB - Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.

KW - Cell biology

KW - Flexion instability

KW - Molecular genetics

KW - Revision total knee arthroplasty

KW - Total knee arthroplasty

UR - http://www.scopus.com/inward/record.url?scp=85035090472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035090472&partnerID=8YFLogxK

U2 - 10.1016/j.ygeno.2017.11.001

DO - 10.1016/j.ygeno.2017.11.001

M3 - Article

C2 - 29174847

AN - SCOPUS:85035090472

JO - Genomics

JF - Genomics

SN - 0888-7543

ER -