Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: Identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G)

Hideyuki Aizawa, Nives Zimmermann, Patricia E. Carrigan, James J. Lee, Marc E. Rothenberg, Bruce S. Bochner

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We recently identified a novel human sialic acid binding immunoglobulin-like lectin, Siglec-8, using mRNA from human eosinophils. To search for a mouse Siglec (mSiglec) ortholog of Siglec-8 and other mouse Siglec paralogs, we conducted public database searches with cDNA sequences of human Siglec-5 to -10 and identified two novel mSiglecs. One has significant sequence identity to human Siglec-5 and is a splice variant of mSiglec-F. The other has greatest sequence identity to human Siglec-10 (mSiglec-G). Both mSiglecs have extracellular Ig-like domains and intracellular tyrosine-based motifs. To determine whether these mSiglecs were relevant to mouse eosinophils, RT-PCR and Northern blot analysis were performed. We detected expression of mSiglec-5 (or -F), -10, and -E mRNA in purified mouse eosinophils, but Northern blot data comparing expression in tissues from normal, IL-5 transgenic, and allergen-sensitized and -challenged mice suggest that mSiglec-10 is probably most relevant to mouse eosinophils.

Original languageEnglish (US)
Pages (from-to)521-530
Number of pages10
JournalGenomics
Volume82
Issue number5
DOIs
StatePublished - Nov 1 2003

Keywords

  • CD33
  • Immunoglobulin superfamily
  • Immunoreceptor tyrosine-based inhibitory motif (ITIM)
  • Mouse eosinophils
  • Sialoadhesin
  • Siglec
  • mSiglec

ASJC Scopus subject areas

  • Genetics

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