Modulation of peripheral cytotoxic cells and ictogenesis in a model of seizures

Nicola Marchi, Aaron J. Johnson, Vikram Puvenna, Holly L. Johnson, William Tierney, Chaitali Ghosh, Luca Cucullo, Paolo F. Fabene, Damir Janigro

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Purpose: A link between seizure susceptibility, blood-brain barrier (BBB) failure, and the activation of peripheral white blood cells has been recently proposed. However, the molecular players involved in this cascade of events are unknown. We tested the hypothesis that immunosupression by splenectomy or lack of perforin, a downstream factor of natural killer (NK) and cytotoxic T cells, could reduce seizure onset. Methods: Pilocarpine was used to induce seizures in adult rats wild-type and perforin-deficient mice. Splenectomy was performed prior to pilocarpine injection. Seizure onset was evaluated by electroencephalography (EEG) and joint time-frequency analysis. Spleens from control and pilocarpine-treated groups were analyzed for anatomical changes and CD3+ cell content. BBB damage was assessed by measuring albumin parenchymal extravasation. Fluorescence-activated cell sorting (FACS) analysis was performed on spleen and brain tissue of wild-type and perforin-deficient mice treated, or not, with pilocarpine. Key Findings: Splenectomy significantly reduced seizure-associated mortality. Histologic analysis of the spleens exposed to pilocarpine revealed altered white and red pulp anatomy and an increase in CD3+ T cells. Onset of status epilepticus (SE) and mortality were significantly decreased in perforin-deficient mice. Pilocarpine significantly increased spleen NK 1.1 and CD8+ cell percentage; in contrast, the brain inflammatory cell profile remained unchanged at the time of pilocarpine SE. BBB damage was reduced in the perforin-deficient pilocarpine-treated mice. Significance: Immunosuppressant maneuvers such as splenectomy or lack of perforin decrease the onset or the severity of pilocarpine SE. Our results suggest that cytotoxic lymphocytes, and specifically the cytolytic factor perforin, may be key molecular players involved in the axis between peripheral intravascular inflammation and seizures.

Original languageEnglish (US)
Pages (from-to)1627-1634
Number of pages8
JournalEpilepsia
Volume52
Issue number9
DOIs
StatePublished - Sep 2011

Keywords

  • Blood-brain barrier
  • Natural killer cells
  • Perforin
  • Seizures
  • T cells

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Modulation of peripheral cytotoxic cells and ictogenesis in a model of seizures'. Together they form a unique fingerprint.

Cite this