Milk-derived extracellular vesicles for therapeutic delivery of small interfering RNAs

Akiko Matsuda, Tushar C Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

As endogenous biological nanoparticles capable of uptake by cells, extracellular vesicles (EVs) have the capacity to deliver their RNA cargo to recipient cells. The use of EVs as a drug delivery system remains in its infancy, and there are several barriers to the use of EV for this purpose. Amongst these is the need to ensure that adequate amounts of EV are available. The use of milk-derived EV provides a scalable approach and loading of these EVs with RNA is possible with the use of chemical transfection reagents. This method describes the use of milk-derived EV for delivery of small interfering RNA. These EVs were shown to be taken up by hepatocellular carcinoma cells in vitro, with a reduction in the expression of target gene.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages187-197
Number of pages11
Volume1740
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1740
ISSN (Print)1064-3745

Keywords

  • Drug delivery
  • Drug loading method
  • Extracellular vesicles
  • Lipofection
  • Milk
  • siRNA
  • Ultracentrifugation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Matsuda, A., & Patel, T. C. (2018). Milk-derived extracellular vesicles for therapeutic delivery of small interfering RNAs. In Methods in Molecular Biology (Vol. 1740, pp. 187-197). (Methods in Molecular Biology; Vol. 1740). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7652-2_15