MicroRNAs and the regulation of vector tropism

Elizabeth J. Kelly, Stephen J Russell

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Despite being small (∼22 nt) microRNAs (miRNAs) profoundly influence tissue-specific gene expression by interacting with complementary target sequences in cellular messenger RNAs, impairing their translation or marking them for early destruction. Recent work has shown that tissue-specific miRNAs offer a versatile target that can be exploited to control the tropisms of gene expression vectors and of replication-competent viruses. The principle of incorporating miRNA targets into vector genomes to control their tropisms was first demonstrated for nonreplicating lentiviral and adenoviral vectors, with subsequent extension of these studies to replication-competent (oncolytic) picornaviruses, rhabdoviruses, and adenoviruses. In contrast to previous targeting approaches, miRNA targeting looks set to be applicable across the entire spectrum of viruses and gene expression vectors. Here we provide a critique of the literature relevant to this new and rapidly developing field of endeavor. We also examine the possibility of engineering viruses for expression of tropism-regulating miRNAs.

Original languageEnglish (US)
Pages (from-to)409-416
Number of pages8
JournalMolecular Therapy
Volume17
Issue number3
DOIs
StatePublished - 2009

Fingerprint

Tropism
MicroRNAs
Gene Expression
Rhabdoviridae
Viruses
Picornaviridae
Virus Replication
Adenoviridae
Genome
Messenger RNA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Drug Discovery
  • Pharmacology

Cite this

MicroRNAs and the regulation of vector tropism. / Kelly, Elizabeth J.; Russell, Stephen J.

In: Molecular Therapy, Vol. 17, No. 3, 2009, p. 409-416.

Research output: Contribution to journalArticle

Kelly, Elizabeth J. ; Russell, Stephen J. / MicroRNAs and the regulation of vector tropism. In: Molecular Therapy. 2009 ; Vol. 17, No. 3. pp. 409-416.
@article{7588b01c3c69496b92945e6244c4cc6e,
title = "MicroRNAs and the regulation of vector tropism",
abstract = "Despite being small (∼22 nt) microRNAs (miRNAs) profoundly influence tissue-specific gene expression by interacting with complementary target sequences in cellular messenger RNAs, impairing their translation or marking them for early destruction. Recent work has shown that tissue-specific miRNAs offer a versatile target that can be exploited to control the tropisms of gene expression vectors and of replication-competent viruses. The principle of incorporating miRNA targets into vector genomes to control their tropisms was first demonstrated for nonreplicating lentiviral and adenoviral vectors, with subsequent extension of these studies to replication-competent (oncolytic) picornaviruses, rhabdoviruses, and adenoviruses. In contrast to previous targeting approaches, miRNA targeting looks set to be applicable across the entire spectrum of viruses and gene expression vectors. Here we provide a critique of the literature relevant to this new and rapidly developing field of endeavor. We also examine the possibility of engineering viruses for expression of tropism-regulating miRNAs.",
author = "Kelly, {Elizabeth J.} and Russell, {Stephen J}",
year = "2009",
doi = "10.1038/mt.2008.288",
language = "English (US)",
volume = "17",
pages = "409--416",
journal = "Molecular Therapy",
issn = "1525-0016",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - MicroRNAs and the regulation of vector tropism

AU - Kelly, Elizabeth J.

AU - Russell, Stephen J

PY - 2009

Y1 - 2009

N2 - Despite being small (∼22 nt) microRNAs (miRNAs) profoundly influence tissue-specific gene expression by interacting with complementary target sequences in cellular messenger RNAs, impairing their translation or marking them for early destruction. Recent work has shown that tissue-specific miRNAs offer a versatile target that can be exploited to control the tropisms of gene expression vectors and of replication-competent viruses. The principle of incorporating miRNA targets into vector genomes to control their tropisms was first demonstrated for nonreplicating lentiviral and adenoviral vectors, with subsequent extension of these studies to replication-competent (oncolytic) picornaviruses, rhabdoviruses, and adenoviruses. In contrast to previous targeting approaches, miRNA targeting looks set to be applicable across the entire spectrum of viruses and gene expression vectors. Here we provide a critique of the literature relevant to this new and rapidly developing field of endeavor. We also examine the possibility of engineering viruses for expression of tropism-regulating miRNAs.

AB - Despite being small (∼22 nt) microRNAs (miRNAs) profoundly influence tissue-specific gene expression by interacting with complementary target sequences in cellular messenger RNAs, impairing their translation or marking them for early destruction. Recent work has shown that tissue-specific miRNAs offer a versatile target that can be exploited to control the tropisms of gene expression vectors and of replication-competent viruses. The principle of incorporating miRNA targets into vector genomes to control their tropisms was first demonstrated for nonreplicating lentiviral and adenoviral vectors, with subsequent extension of these studies to replication-competent (oncolytic) picornaviruses, rhabdoviruses, and adenoviruses. In contrast to previous targeting approaches, miRNA targeting looks set to be applicable across the entire spectrum of viruses and gene expression vectors. Here we provide a critique of the literature relevant to this new and rapidly developing field of endeavor. We also examine the possibility of engineering viruses for expression of tropism-regulating miRNAs.

UR - http://www.scopus.com/inward/record.url?scp=61649088631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61649088631&partnerID=8YFLogxK

U2 - 10.1038/mt.2008.288

DO - 10.1038/mt.2008.288

M3 - Article

C2 - 19107117

AN - SCOPUS:61649088631

VL - 17

SP - 409

EP - 416

JO - Molecular Therapy

JF - Molecular Therapy

SN - 1525-0016

IS - 3

ER -