MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion

Guntulu Ak, Sandra C. Tomaszek, Farhad Kosari, Muzaffer Metintas, James R. Jett, Selma Metintas, Huseyin Yildirim, Emine Dundar, Jie Dong, Marie Christine Aubry, Dennis A. Wigle, Charles F. Thomas

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28 Scopus citations

Abstract

Introduction: We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion. Methods: Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols. Results: We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway. Conclusions: Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.

Original languageEnglish (US)
Article number635748
JournalBioMed Research International
Volume2015
DOIs
StatePublished - 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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    Ak, G., Tomaszek, S. C., Kosari, F., Metintas, M., Jett, J. R., Metintas, S., Yildirim, H., Dundar, E., Dong, J., Aubry, M. C., Wigle, D. A., & Thomas, C. F. (2015). MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion. BioMed Research International, 2015, [635748]. https://doi.org/10.1155/2015/635748