MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion

Guntulu Ak, Sandra C. Tomaszek, Farhad Kosari, Muzaffer Metintas, James R. Jett, Selma Metintas, Huseyin Yildirim, Emine Dundar, Jie Dong, Marie Christine Aubry, Dennis A Wigle, Charles F. Thomas

Research output: Contribution to journalArticle

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Abstract

Introduction: We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion. Methods: Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols. Results: We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway. Conclusions: Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.

Original languageEnglish (US)
Article number635748
JournalBioMed Research International
Volume2015
DOIs
StatePublished - 2015

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Asbestos
Pleural Effusion
MicroRNAs
Messenger RNA
Tissue
Mesothelioma
Up-Regulation
Genes
Malignant Mesothelioma
Incidence

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion. / Ak, Guntulu; Tomaszek, Sandra C.; Kosari, Farhad; Metintas, Muzaffer; Jett, James R.; Metintas, Selma; Yildirim, Huseyin; Dundar, Emine; Dong, Jie; Aubry, Marie Christine; Wigle, Dennis A; Thomas, Charles F.

In: BioMed Research International, Vol. 2015, 635748, 2015.

Research output: Contribution to journalArticle

Ak, G, Tomaszek, SC, Kosari, F, Metintas, M, Jett, JR, Metintas, S, Yildirim, H, Dundar, E, Dong, J, Aubry, MC, Wigle, DA & Thomas, CF 2015, 'MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion', BioMed Research International, vol. 2015, 635748. https://doi.org/10.1155/2015/635748
Ak, Guntulu ; Tomaszek, Sandra C. ; Kosari, Farhad ; Metintas, Muzaffer ; Jett, James R. ; Metintas, Selma ; Yildirim, Huseyin ; Dundar, Emine ; Dong, Jie ; Aubry, Marie Christine ; Wigle, Dennis A ; Thomas, Charles F. / MicroRNA and mRNA features of malignant pleural mesothelioma and benign asbestos-related pleural effusion. In: BioMed Research International. 2015 ; Vol. 2015.
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AU - Thomas, Charles F.

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N2 - Introduction: We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion. Methods: Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols. Results: We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway. Conclusions: Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.

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