MicroRNA and mRNA cargo of extracellular vesicles from porcine adipose tissue-derived mesenchymal stem cells

Alfonso Eirin, Scott M. Riester, Xiang Yang Zhu, Hui Tang, Jared M. Evans, Daniel O'Brien, Andre J van Wijnen, Lilach O Lerman

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Mesenchymal stromal/stem cells (MSCs) are clinically useful for cell-based therapy, but concerns regarding their ability to replicate limit their human application. MSCs release extracellular vesicles (EVs) that mediate at least in part the paracrine effects of the parental cells. To understand the molecular basis of their biological properties, we characterized the RNA cargo of EVs from porcine adipose-tissue derived MSCs. Comprehensive characterization of mRNA and miRNA gene expression using high-throughput RNA sequencing (RNA-seq) revealed that EVs are selectively enriched for distinct classes of RNAs. For example, EVs preferentially express mRNA for transcription factors (e.g. MDFIC, POU3F1, NRIP1) and genes involved in angiogenesis (e.g. HGF, HES1, TCF4) and adipogenesis (e.g. CEBPA, KLF7). EVs also express Golgi apparatus genes (ARRB1, GOLGA4) and genes involved in TGF-β signaling. In contrast, mitochondrial, calcium signaling, and cytoskeleton genes are selectively excluded from EVs, possibly because these genes remain sequestered in organelles or intracellular compartments. RNA-seq generated reads for at least 386 annotated miRNAs, but only miR148a, miR532-5p, miR378, and let-7f were enriched in EVs compared to MSCs. Gene ontology analysis indicates that these miRNAs target transcription factors and genes that participate in several cellular pathways, including angiogenesis, cellular transport, apoptosis, and proteolysis. Our data suggest that EVs transport gene regulatory information to modulate angiogenesis, adipogenesis, and other cell pathways in recipient cells. These observations may contribute to development of regenerative strategies using EVs to overcome potential complications of cell-based therapy.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalGene
Volume551
Issue number1
DOIs
StatePublished - Nov 1 2014

Fingerprint

MicroRNAs
Mesenchymal Stromal Cells
Adipose Tissue
Swine
Messenger RNA
Genes
Adipogenesis
Cell- and Tissue-Based Therapy
Transcription Factors
RNA
RNA Sequence Analysis
Extracellular Vesicles
High-Throughput Nucleotide Sequencing
Gene Ontology
Calcium Signaling
Golgi Apparatus
Regulator Genes
Cytoskeleton
Organelles
Proteolysis

Keywords

  • Exosomes
  • Extracellular vesicles
  • Gene expression
  • Mesenchymal stem cells
  • Microvesicles
  • MiRNA
  • Next generation sequencing (NGS)
  • RNASeq

ASJC Scopus subject areas

  • Genetics

Cite this

MicroRNA and mRNA cargo of extracellular vesicles from porcine adipose tissue-derived mesenchymal stem cells. / Eirin, Alfonso; Riester, Scott M.; Zhu, Xiang Yang; Tang, Hui; Evans, Jared M.; O'Brien, Daniel; van Wijnen, Andre J; Lerman, Lilach O.

In: Gene, Vol. 551, No. 1, 01.11.2014, p. 55-64.

Research output: Contribution to journalArticle

Eirin, Alfonso ; Riester, Scott M. ; Zhu, Xiang Yang ; Tang, Hui ; Evans, Jared M. ; O'Brien, Daniel ; van Wijnen, Andre J ; Lerman, Lilach O. / MicroRNA and mRNA cargo of extracellular vesicles from porcine adipose tissue-derived mesenchymal stem cells. In: Gene. 2014 ; Vol. 551, No. 1. pp. 55-64.
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