TY - JOUR
T1 - Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells
AU - Linden, David R.
AU - El-Fakahany, Esam E.
N1 - Funding Information:
The authors would like to thank Ms. Kristin Schreiber for her valuable input to the development of these studies. This work was supported by the NIH grant NS25743.
PY - 2002/2/1
Y1 - 2002/2/1
N2 - Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca2+ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.
AB - Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca2+ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.
KW - 5-HT (5-hydroxytryptamine, serotonin)
KW - Neuromodulation
KW - Nitric oxide (NO) synthase
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U2 - 10.1016/S0014-2999(01)01615-6
DO - 10.1016/S0014-2999(01)01615-6
M3 - Article
C2 - 11834246
AN - SCOPUS:0036468372
SN - 0014-2999
VL - 436
SP - 53
EP - 56
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-2
ER -