Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells

David R. Linden, Esam E. El-Fakahany

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca2+ and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.

Original languageEnglish (US)
Pages (from-to)53-56
Number of pages4
JournalEuropean Journal of Pharmacology
Volume436
Issue number1-2
DOIs
StatePublished - Feb 1 2002

Keywords

  • 5-HT (5-hydroxytryptamine, serotonin)
  • Neuromodulation
  • Nitric oxide (NO) synthase

ASJC Scopus subject areas

  • Pharmacology

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