Microarray analysis on gene regulation by estrogen, progesterone and tamoxifen in human endometrial stromal cells

Chun E. Ren, Xueqiong Zhu, Jinping Li, Christian Lyle, Sean Dowdy, Karl C. Podratz, David Byck, Hai Bin Chen, Shi Wen Jiang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies.

Original languageEnglish (US)
Pages (from-to)5864-5885
Number of pages22
JournalInternational journal of molecular sciences
Volume16
Issue number3
DOIs
StatePublished - Mar 13 2015

Keywords

  • Estrogen
  • Progesterone
  • Stroma
  • Tamoxifen
  • Transcription
  • Uterus

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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