Metabolomics in stem cell biology research

Zhen Sun, Jing Zhao, Hua Yu, Chenyang Zhang, Hu Li, Zhongda Zeng, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Stem cell research has been greatly facilitated by comprehensive and integrative multi-omics studies. As a unique approach of functional analysis, metabolomics measures many metabolites and activities of metabolic pathways which can directly indicate cellular energetic status, cell proliferation and fitness, and stem cell fate choices such as self-renewal versus differentiation. Here we describe the methods of applying metabolomics, 13 C-labeled glucose and glutamine tracing with mouse embryonic stem cells (ES cells), metabolite analysis using mass spectrometry tools, and the following statistical and computational modeling analysis. Integration of these methods into the more common gene expression and epigenetics analysis toolbox will help to generate a more complete picture and in-depth understanding of one's stem cells of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages321-330
Number of pages10
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1975
ISSN (Print)1064-3745

Fingerprint

Stem Cell Research
Metabolomics
Cell Biology
Stem Cells
Metabolic Networks and Pathways
Glutamine
Epigenomics
Mass Spectrometry
Cell Proliferation
Gene Expression
Glucose
Mouse Embryonic Stem Cells

Keywords

  • C-tracing analysis
  • Mass spectroscopy
  • Metabolic pathway
  • Metabolite
  • Metabolomics
  • Statistical analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sun, Z., Zhao, J., Yu, H., Zhang, C., Li, H., Zeng, Z., & Zhang, J. (2019). Metabolomics in stem cell biology research. In Methods in Molecular Biology (pp. 321-330). (Methods in Molecular Biology; Vol. 1975). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9224-9_15

Metabolomics in stem cell biology research. / Sun, Zhen; Zhao, Jing; Yu, Hua; Zhang, Chenyang; Li, Hu; Zeng, Zhongda; Zhang, Jin.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 321-330 (Methods in Molecular Biology; Vol. 1975).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sun, Z, Zhao, J, Yu, H, Zhang, C, Li, H, Zeng, Z & Zhang, J 2019, Metabolomics in stem cell biology research. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1975, Humana Press Inc., pp. 321-330. https://doi.org/10.1007/978-1-4939-9224-9_15
Sun Z, Zhao J, Yu H, Zhang C, Li H, Zeng Z et al. Metabolomics in stem cell biology research. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 321-330. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9224-9_15
Sun, Zhen ; Zhao, Jing ; Yu, Hua ; Zhang, Chenyang ; Li, Hu ; Zeng, Zhongda ; Zhang, Jin. / Metabolomics in stem cell biology research. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 321-330 (Methods in Molecular Biology).
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