Metabolomics in stem cell biology research

Zhen Sun, Jing Zhao, Hua Yu, Chenyang Zhang, Hu Li, Zhongda Zeng, Jin Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Stem cell research has been greatly facilitated by comprehensive and integrative multi-omics studies. As a unique approach of functional analysis, metabolomics measures many metabolites and activities of metabolic pathways which can directly indicate cellular energetic status, cell proliferation and fitness, and stem cell fate choices such as self-renewal versus differentiation. Here we describe the methods of applying metabolomics, 13 C-labeled glucose and glutamine tracing with mouse embryonic stem cells (ES cells), metabolite analysis using mass spectrometry tools, and the following statistical and computational modeling analysis. Integration of these methods into the more common gene expression and epigenetics analysis toolbox will help to generate a more complete picture and in-depth understanding of one's stem cells of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages321-330
Number of pages10
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1975
ISSN (Print)1064-3745

Keywords

  • C-tracing analysis
  • Mass spectroscopy
  • Metabolic pathway
  • Metabolite
  • Metabolomics
  • Statistical analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Sun, Z., Zhao, J., Yu, H., Zhang, C., Li, H., Zeng, Z., & Zhang, J. (2019). Metabolomics in stem cell biology research. In Methods in Molecular Biology (pp. 321-330). (Methods in Molecular Biology; Vol. 1975). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9224-9_15