Metabolites and microbial composition of stool of women with fecal incontinence: Study design and methods

Lily A. Arya, Holly E. Richter, Eric Jelovsek, Marie Gantz, Sara Cichowski, Halina Zyczynski, Keisha Dyer, Nazema Siddiqui, Cassandra Carberry, Corey Broeckling, Casey Morrow, Purna Kashyap, Susie Meikle

Research output: Contribution to journalArticle

Abstract

Aims: This paper aims to report the rationale, design, and the specific methodology of an ongoing nested observational study that will determine the association of the metabolite and microbial composition of stool with fecal incontinence (FI). Methods: Eligible cases are participants with FI enrolled in the Controlling Anal Incontinence in women by Performing Anal Exercises with Biofeedback or Loperamide (CAPABLe) trial, a Pelvic Floor Disorders Network trial across eight clinical centers in the United States. Women of similar age without FI in the last year served as controls. All subject collected stool samples at the baseline and 24-week visit at home using a standardized collection method. Samples were shipped to and stored at centralized laboratories. Results: Specimen collection commenced December 2014 and was completed in May 2016. Fecal water and DNA has been extracted and is currently being analyzed by targeted metabolic profiling for stool metabolites and 16S rRNA gene sequencing for stool microbiota. Conclusions: This article describes the rationale and design of a study that could provide a paradigm shift for the treatment of FI in women.

Original languageEnglish (US)
Pages (from-to)634-641
Number of pages8
JournalNeurourology and Urodynamics
Volume37
Issue number2
DOIs
StatePublished - Feb 1 2018

Keywords

  • butyrate
  • fecal incontinence
  • fecal urgency
  • metabolites
  • microbiota

ASJC Scopus subject areas

  • Clinical Neurology
  • Urology

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  • Cite this

    Arya, L. A., Richter, H. E., Jelovsek, E., Gantz, M., Cichowski, S., Zyczynski, H., Dyer, K., Siddiqui, N., Carberry, C., Broeckling, C., Morrow, C., Kashyap, P., & Meikle, S. (2018). Metabolites and microbial composition of stool of women with fecal incontinence: Study design and methods. Neurourology and Urodynamics, 37(2), 634-641. https://doi.org/10.1002/nau.23360