@article{f2c1be8f418144c79886076de51090e4,
title = "Metabolites and microbial composition of stool of women with fecal incontinence: Study design and methods",
abstract = "Aims: This paper aims to report the rationale, design, and the specific methodology of an ongoing nested observational study that will determine the association of the metabolite and microbial composition of stool with fecal incontinence (FI). Methods: Eligible cases are participants with FI enrolled in the Controlling Anal Incontinence in women by Performing Anal Exercises with Biofeedback or Loperamide (CAPABLe) trial, a Pelvic Floor Disorders Network trial across eight clinical centers in the United States. Women of similar age without FI in the last year served as controls. All subject collected stool samples at the baseline and 24-week visit at home using a standardized collection method. Samples were shipped to and stored at centralized laboratories. Results: Specimen collection commenced December 2014 and was completed in May 2016. Fecal water and DNA has been extracted and is currently being analyzed by targeted metabolic profiling for stool metabolites and 16S rRNA gene sequencing for stool microbiota. Conclusions: This article describes the rationale and design of a study that could provide a paradigm shift for the treatment of FI in women.",
keywords = "butyrate, fecal incontinence, fecal urgency, metabolites, microbiota",
author = "Arya, {Lily A.} and Richter, {Holly E.} and Eric Jelovsek and Marie Gantz and Sara Cichowski and Halina Zyczynski and Keisha Dyer and Nazema Siddiqui and Cassandra Carberry and Corey Broeckling and Casey Morrow and Purna Kashyap and Susie Meikle",
note = "Funding Information: National Institutes of Health Office of Research on Women{\textquoteright}s Health. Funding Information: The authors would like to acknowledge the contributions of Tiffany Weir, PhD, Colorado State University Proteomics and Metabolomics Facility, in preparing and storing stool specimens. Dr. Kashyap{\textquoteright}s effort was supported in part by NIHK08DK100638. The authors gratefully acknowledge the funding by The National Institutes of Health Office of Research on Women{\textquoteright}s Health and Eunice Kennedy Shriver National Institute of Child Health and Human Development (2U01HD41249, 2U10HD41250, 2U10HD41261, 2U10HD 41267, 1U10HD54136, 1U10HD54214, 1U10HD54215, and 1U10 HD54241). Funding Information: The authors would like to acknowledge the contributions of Tiffany Weir, PhD, Colorado State University Proteomics and Metabolomics Facility, in preparing and storing stool specimens. Dr. Kashyap's effort was supported in part by NIHK08DK100638. The authors gratefully acknowledge the funding by The National Institutes of Health Office of Research on Women's Health and Eunice Kennedy Shriver National Institute of Child Health and Human Development (2U01HD41249, 2U10HD41250, 2U10HD41261, 2U10HD41267, 1U10HD54136, 1U10HD54214, 1U10HD54215, and 1U10 HD54241). Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",
year = "2018",
month = feb,
doi = "10.1002/nau.23360",
language = "English (US)",
volume = "37",
pages = "634--641",
journal = "Neurourology and Urodynamics",
issn = "0733-2467",
publisher = "Wiley-Liss Inc.",
number = "2",
}